RPL10A Recombinant monoclonal antibody 85685-1-RR proteintech
$149.00
In stock
SKU
85685-1-RR
| Catalog Number | 85685-1-RR |
| Synonyms | 60S ribosomal protein L10a, Csa 19, CSA-19, Large ribosomal subunit protein uL1, NEDD 6 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | K-562 cells, Jurkat cells, HepG2 cells, HeLa cells, mouse spleen tissue, rat spleen tissue, fetal human brain tissue |
| Tested Applications — Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | K-562 cells, Jurkat cells, HepG2 cells, HeLa cells, mouse spleen tissue, rat spleen tissue, fetal human brain tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag10009 Product name: Recombinant human RPL10A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC006791 Sequence: MSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQMKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY Predict reactive species |
| Full Name | ribosomal protein L10a |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC006791 |
| Gene Symbol | RPL10A |
| Gene ID (NCBI) | 4736 |
| RRID | AB_3744292 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P62906 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for RPL10A antibody 85685-1-RR | Download protocol |
| IF protocol for RPL10A antibody 85685-1-RR | Download protocol |
| IHC protocol for RPL10A antibody 85685-1-RR | Download protocol |
| WB protocol for RPL10A antibody 85685-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 243031C10 |
| Conjugation | Unconjugated |
RPL10A (Ribosomal Protein L10a) is a component of the 60S subunit of the ribosome and belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. RPL10A is ubiquitously expressed in various tissues, it plays a crucial role in ribosome biogenesis and protein synthesis. In addition to its role in protein synthesis, RPL10A has been implicated in cell proliferation, embryogenesis, and organogenesis. PMID: 29953919 Protocols Product Specific Protocols FC protocol for RPL10A antibody 85685-1-RR Download protocol IF protocol for RPL10A antibody 85685-1-RR Download protocol IHC protocol for RPL10A antibody 85685-1-RR Download protocol WB protocol for RPL10A antibody 85685-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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