RPLP1 Polyclonal antibody 21636-1-AP proteintech
$149.00
In stock
SKU
21636-1-AP
| Catalog Number | 21636-1-AP |
| Synonyms | Large ribosomal subunit protein P1, RPLP 1, RPP1, RRP1 |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, MDA-MB-231 cells |
| Tested Applications — Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, HepG2 cells, MDA-MB-231 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag16268 Product name: Recombinant human RPLP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-114 aa of BC003369 Sequence: MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMGFGLFD Predict reactive species |
| Full Name | ribosomal protein, large, P1 |
| Calculated Molecular Weight | 12 kDa |
| Observed Molecular Weight | 15-20 kDa |
| GenBank Accession Number | BC003369 |
| Gene Symbol | RPLP1 |
| Gene ID (NCBI) | 6176 |
| RRID | AB_2878894 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05386 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| IHC protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| WB protocol for RPLP1 antibody 21636-1-AP | Download protocol |
| pig | WB Virulence RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome. Authors - Longxiang Zhang KD Validated View Article |
| mouse | WB Mol Hum Reprod 60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro. Authors - Zahraa Alali View Article |
| human | WB,IF Nat Commun RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR Authors - Weijing Yang View Article |
| Citations | 6 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
60S acidic ribosomal protein P1 (RRP1) is a 114 amino acid protein, which belongs to the ribosomal protein L12P family. RRP1 plays an important role in the elongation step of protein synthesis. The molecular weight of modified RRP1 is 15-20 kDa. Protocols Product Specific Protocols IF protocol for RPLP1 antibody 21636-1-AP Download protocol IHC protocol for RPLP1 antibody 21636-1-AP Download protocol WB protocol for RPLP1 antibody 21636-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- RPLP1, an NS4B-interacting protein, enhances production of CSFV through promoting translation of viral genome.
Journal: Virulence | Authors: Longxiang Zhang KD Validated | Species: pig | Application: WB - 60S acidic ribosomal protein P1 (RPLP1) is elevated in human endometriotic tissue and in a murine model of endometriosis and is essential for endometriotic epithelial cell survival in vitro.
Journal: Mol Hum Reprod | Authors: Zahraa Alali | Species: mouse | Application: WB - Unveiling the prognostic implications of RPLP1 upregulation in osteosarcoma
Journal: Am J Cancer Res | Authors: Xinhui Du | Species: human | Application: WB,IHC - RPLP1 Is Up-Regulated in Human Adenomyosis and Endometrial Adenocarcinoma Epithelial Cells and Is Essential for Cell Survival and Migration In Vitro
Journal: Int J Mol Sci | Authors: Riley Peterson | Species: human | Application: WB - RPLP1 restricts HIV-1 transcription by disrupting C/EBPβ binding to the LTR
Journal: Nat Commun | Authors: Weijing Yang | Species: human | Application: WB,IF - Ribosome customization and functional diversification among P-stalk proteins regulate late poxvirus protein synthesis
Journal: Cell Rep | Authors: Natalia Khalatyan | Application: WB
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