NOTCH3 Polyclonal antibody 55114-1-AP proteintech
$149.00
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SKU
55114-1-AP
| Catalog Number | 55114-1-AP |
| Synonyms | CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila) |
| Host | Rabbit |
| Reactivity | human, mouse and More (2) |
| Reactivity | human, mouse, rat, goat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A2780 cells, Caco-2 cells, U2OS cells, COLO 320 cells, HeLa cells, K-562 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | A2780 cells, Caco-2 cells, U2OS cells, COLO 320 cells, HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | Notch homolog 3 (Drosophila) |
| Calculated Molecular Weight | 244 kDa |
| Observed Molecular Weight | 250-260 kDa, 100 kDa |
| GenBank Accession Number | NM_000435 |
| Gene Symbol | NOTCH3 |
| Gene ID (NCBI) | 4854 |
| RRID | AB_10858393 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UM47 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for NOTCH3 antibody 55114-1-AP | Download protocol |
| IHC protocol for NOTCH3 antibody 55114-1-AP | Download protocol |
| IP protocol for NOTCH3 antibody 55114-1-AP | Download protocol |
| WB protocol for NOTCH3 antibody 55114-1-AP | Download protocol |
| mouse | WB Cancer Res Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction. Authors - Shubing Zhang View Article |
| rat,mouse,human | WB,IF Sci Transl Med JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension. Authors - Yu Zhang View Article |
| rat | WB Biomaterials Laminin-521 promotes quiescence in isolated stellate cells from rat liver. Authors - Friederike Rohn View Article |
| human | WB Oncogene Notch3 promotes prostate cancer-induced bone lesion development via MMP-3. Authors - Sourik S Ganguly KD Validated View Article |
| Nikolett (Verified Customer) (10-14-2025) | AB works well for Western Blott application on human lung cancer cell lines after overnight incubation. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human Lung Cancer Cell Lines |
| Citations | 76 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048). Protocols Product Specific Protocols IF protocol for NOTCH3 antibody 55114-1-AP Download protocol IHC protocol for NOTCH3 antibody 55114-1-AP Download protocol IP protocol for NOTCH3 antibody 55114-1-AP Download protocol WB protocol for NOTCH3 antibody 55114-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
Journal: Cell Metab | Authors: Xiuying Zhong | Species: mouse | Application: WB - JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.
Journal: Sci Transl Med | Authors: Yu Zhang | Species: rat,mouse,human | Application: WB,IF - METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.
Journal: Mol Ther | Authors: Ling Jiang | Species: mouse | Application: WB - Laminin-521 promotes quiescence in isolated stellate cells from rat liver.
Journal: Biomaterials | Authors: Friederike Rohn | Species: rat | Application: WB - Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.
Journal: Cancer Res | Authors: Shubing Zhang | Species: mouse | Application: WB - Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.
Journal: Oncogene | Authors: Sourik S Ganguly KD Validated | Species: human | Application: WB - Combined therapy with DR5-targeting antibody-drug conjugate and CDK inhibitors as a strategy for advanced colorectal cancer
Journal: Cell Rep Med | Authors: Dongdong Zhou | Species: human | Application: WB - Laminin-521 promotes quiescence in isolated stellate cells from rat liver.
Journal: Biomaterials | Authors: Friederike Rohn | Species: rat | Application: WB - NICD3 mediates pro-angiogenic effects through SMAD3/TGFBI axis in colorectal cancer.
Journal: Cell Death Dis | Authors: Rui An - FPR3 reprograms glycolytic metabolism and stemness in gastric cancer via calcium-NFATc1 pathway
Journal: Cancer Lett | Authors: Lingzhi Wang | Species: human | Application: WB,IF - UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185.
Journal: Int J Biol Sci | Authors: Zhikun Zheng | Species: human | Application: WB - Upregulated MMP28 in Hepatocellular Carcinoma Promotes Metastasis via Notch3 Signaling and Predicts Unfavorable Prognosis.
Journal: Int J Biol Sci | Authors: Jiangfan Zhou | Species: human | Application: WB - Upregulated MMP28 in Hepatocellular Carcinoma Promotes Metastasis via Notch3 Signaling and Predicts Unfavorable Prognosis.
Journal: Int J Biol Sci | Authors: Jiangfan Zhou | Species: human | Application: WB - Reduced SUMOylation impairs NOTCH3 signaling and cell survival in the pathogenesis of CADASIL.
Journal: Cell Commun Signal | Authors: Lijun Long | Species: human,mouse | Application: WB,IP - METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.
Journal: Mol Ther | Authors: Ling Jiang | Species: mouse | Application: WB - Unraveling the neuroprotective mechanism of gastrodin against CRS-induced depression in adult male mice via Notch3: Integration of proteomics, transcriptomics, molecular docking, and cellular validation.
Journal: Phytomedicine | Authors: Hongchun Xiang | Species: mouse | Application: WB,IF - Didymin ameliorates ulcerative colitis-associated secondary liver damage by facilitating Notch1 degradation
Journal: Phytomedicine | Authors: Qi Lv | Species: mouse | Application: WB - Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers
Journal: Cell Death Discov | Authors: Zhimin Wang | Species: goat | Application: WB - LncRNA-Gm9866 promotes liver fibrosis by activating TGFβ/Smad signaling via targeting Fam98b
Journal: J Transl Med | Authors: Xiaomin Liao | Species: mouse | Application: WB - Notch3 enhances the synergistic effect of all-trans retinoic acid and calcipotriol in pancreatic stellate cell activation
Journal: J Transl Med | Authors: Zheng Lian | Species: mouse | Application: WB - Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.
Journal: Oncogene | Authors: S Zhang | Species: mouse,human | Application: WB,IHC - Lfng-expressing centroacinar cell is a unique cell-of-origin for p53 deficient pancreatic cancer
Journal: Oncogene | Authors: Wen-Cheng Chung | Species: mouse | Application: IHC - Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.
Journal: Oncogene | Authors: Sourik S Ganguly | Species: human | Application: WB - RNLS hypomethylation as a potential epigenetic marker of lung cancer metastasis: an epigenome-wide association study.
Journal: Br J Cancer | Authors: Zhuangjie Guo | Species: human | Application: WB - Environmental nano-particulates induced pre-implantation embryonic toxicity in pluripotent stem cell-derived blastoids
Journal: Environ Pollut | Authors: Gaojing Liu | Species: human | Application: IF - Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer
Journal: Front Immunol | Authors: Yanling Zhou | Species: human | Application: IHC - NOTCH3 attenuates cytotoxicity via RBPJ-dependent PVR upregulation to influence immunotherapy outcomes in colorectal cancer.
Journal: Front Immunol | Authors: Qi Ma | Species: mouse - Wutou decoction ameliorates rheumatoid arthritis through inhibiting hypoxia-induced invasive phenotype of fibroblast-like synoviocytes mediated by the Notch pathway.
Journal: J Ethnopharmacol | Authors: Weiji Lin | Species: rat, human | Application: WB,IHC - Role of notch signaling pathway in Muc5ac secretion induced by atmospheric PM2.5 in rats.
Journal: Ecotoxicol Environ Saf | Authors: Ying Liu | Species: rat | Application: WB - The effect of Notch signal pathway on PM2.5-induced Muc5ac in Beas-2B cells.
Journal: Ecotoxicol Environ Saf | Authors: Ying Liu | Species: human | Application: WB - Role of notch signaling pathway in Muc5ac secretion induced by atmospheric PM2.5 in rats.
Journal: Ecotoxicol Environ Saf | Authors: Ying Liu | Species: rat | Application: WB - Exosomal Notch3 from high glucose-stimulated endothelial cells regulates vascular smooth muscle cells calcification/aging.
Journal: Life Sci | Authors: Xiao Lin | Species: human | Application: WB - GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma.
Journal: Life Sci | Authors: Wei Wang | Species: human | Application: WB - Systems medicine approach unravels MMP2 and NOTCH3 as key mediators of cigarette smoke-induced airway remodelling in COPD.
Journal: Comput Biol Med | Authors: Anupama Dubey - Mechanism of the Traditional Chinese Medicine Simiao Biejia Decoction Improves the Diabetes Mellitus-Induced Erectile Dysfunction in Rats
Journal: Drug Des Devel Ther | Authors: Yuanyuan Liu | Species: human | Application: WB - Genetic depletion or pharmacological degradation of EZH2 attenuates renal fibrosis via suppressing Notch signaling.
Journal: Clin Epigenetics | Authors: Chao Yu | Species: mouse | Application: WB,IF - Genetic depletion or pharmacological degradation of EZH2 attenuates renal fibrosis via suppressing Notch signaling.
Journal: Clin Epigenetics | Authors: Chao Yu | Species: mouse | Application: WB,IF - Bletilla striata polysaccharide-mediated trained immunity drives the hematopoietic progenitors' expansion and myelopoiesis
Journal: Int Immunopharmacol | Authors: Jiangtao Zhang | Species: mouse | Application: IF - Androgen receptor inhibitor ameliorates pulmonary arterial hypertension by enhancing the apoptosis level through suppressing the Notch3/Hes5 pathway
Journal: Front Pharmacol | Authors: Jiayan Sun | Species: rat,mouse | Application: WB,IF - Piezo1-mediated fluid shear stress promotes OPG and inhibits RANKL via NOTCH3 in MLO-Y4 osteocytes.
Journal: Channels (Austin) | Authors: Zhongcheng Liu | Species: mouse | Application: WB - Enhanced Notch3 signaling contributes to pulmonary emphysema in a Murine Model of Marfan syndrome.
Journal: Sci Rep | Authors: Kathryn Jespersen | Species: mouse | Application: IF - Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis.
Journal: Sci Rep | Authors: Qi Ding | Species: human | Application: WB,IHC,IF - Enhanced Notch3 signaling contributes to pulmonary emphysema in a Murine Model of Marfan syndrome.
Journal: Sci Rep | Authors: Kathryn Jespersen | Species: mouse | Application: IF - DLL3 regulates the migration and invasion of small cell lung cancer by modulating Snail.
Journal: Cancer Sci | Authors: Megumi Furuta | Species: human | Application: WB - Selective Targeting of Class I Histone Deacetylases in a Model of Human Osteosarcoma.
Journal: Cancers (Basel) | Authors: Haydee M Torres | Species: human | Application: WB - Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia.
Journal: Neoplasia | Authors: Wen-Cheng Chung | Species: mouse | Application: WB,IHC - Lunatic Fringe and p53 Cooperatively Suppress Mesenchymal Stem-Like Breast Cancer.
Journal: Neoplasia | Authors: Wen-Cheng Chung | Species: mouse | Application: WB,IHC - Tumor-suppressive activity of Lunatic Fringe in prostate through differential modulation of Notch receptor activation.
Journal: Neoplasia | Authors: Shubing Zhang | Species: mouse | Application: WB - SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing
Journal: J Cell Mol Med | Authors: Xi Chen | Species: human | Application: WB - Elabela gene therapy promotes angiogenesis after myocardial infarction.
Journal: J Cell Mol Med | Authors: Liangli Jin | Species: human | Application: WB - Circ_PUM1 promotes the development of endometrial cancer by targeting the miR-136/NOTCH3 pathway.
Journal: J Cell Mol Med | Authors: Zhi-Hong Zong | Species: human | Application: WB - Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma.
Journal: Oncol Rep | Authors: Xiaoyue Huang | Species: human | Application: WB - GHITM regulates malignant phenotype and sensitivity to PD-1 blockade of renal cancer cells via Notch signalling
Journal: J Cell Mol Med | Authors: Shiyu Huang | Species: human | Application: WB - Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway
Journal: Environ Toxicol | Authors: Yuezhu Zhang | Species: rat | Application: WB,IF - C/EBPβ mediates anti-proliferative effects of 1,25(OH)2D on differentiated thyroid carcinoma cells.
Journal: Endocr Relat Cancer | Authors: Feng Xu | Species: human | Application: WB - Notch3 Modulates Cardiac Fibroblast Proliferation, Apoptosis, and Fibroblast to Myofibroblast Transition via Negative Regulation of the RhoA/ROCK/Hif1α Axis.
Journal: Front Physiol | Authors: Jianli Shi | Species: rat | Application: WB - A tumor-suppressive function for Notch3 in the parous mammary gland
Journal: Development | Authors: Wen-Cheng Chung | Species: mouse | Application: WB - Mild hypothermia inhibits the Notch 3 and Notch 4 activation and seizure after stroke in the rat model.
Journal: Pathol Res Pract | Authors: Guo-Shuai Yang | Species: rat | Application: WB,IHC - Huaier suppresses cell viability, migration and invasion in human non-small cell lung cancer via lncRNA DLEU2/miR-212-5p/ELF3 axis
Journal: Int J Med Sci | Authors: Tangwei Wu | Species: human | Application: WB - Antagonistic interaction between Notch3 signaling and CREB/KLF15 pathway in regulating the phenotypic alterations of glomerular parietal epithelial cells in adriamycin-induced nephropathy.
Journal: Exp Cell Res | Authors: Yuqing Zhu | Species: mouse | Application: IF - A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer
Journal: Front Oncol | Authors: Xianyun Xu | Species: human | Application: WB - Notch signaling modulates Fgf23 expression through crosstalk with hypoxia and PTH pathways in osteogenic cells
Journal: Mol Cell Endocrinol | Authors: Yoshihiro Tamamura - hsa_circ_0001741 promotes esophageal squamous cell carcinoma stemness, invasion and migration by sponging miR-491-5p to upregulate NOTCH3 expression.
Journal: Am J Cancer Res | Authors: Lin Li | Species: mouse | Application: IHC - Anoikis patterns via machine learning strategy and experimental verification exhibit distinct prognostic and immune landscapes in melanoma
Journal: Clin Transl Oncol | Authors: Jinfang Liu | Species: human | Application: IHC - Notch signaling via regulation of RB and p-AKT but not PIK3CG contributes to MIA PaCa-2 cell growth and migration to affect pancreatic carcinogenesis.
Journal: Oncol Lett | Authors: Shubing Zhang | Species: human | Application: IHC - Differential Roles of Notch Receptors in Regulating Activation of Intact Adult Mouse Spinal Cord-Derived NSCs.
Journal: Dev Neurobiol | Authors: Juan Li | Species: mouse | Application: IF - Differential Roles of Notch Receptors in Regulating Activation of Intact Adult Mouse Spinal Cord-Derived NSCs.
Journal: Dev Neurobiol | Authors: Juan Li | Species: mouse | Application: IF - RO4929097 inhibits NICD3 to alleviate pulmonary hypertension via blocking Notch3/HIF-2α/FoxM1 signaling pathway
Journal: In Vitro Cell Dev Biol Anim | Authors: Hao Zhu | Species: rat | Application: WB,IF - Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation.
Journal: Stem Cells Dev | Authors: Eva Christine Schumacher | Species: rat | Application: WB - Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer.
Journal: Transl Androl Urol | Authors: Yunhao Qing | Species: human | Application: IHC - PLXND1 from the Plexins family is a prognostic factor promoting colon cancer invasion and metastasis.
Journal: Am J Transl Res | Authors: Wenxing Chen | Species: human | Application: WB - Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway.
Journal: World J Clin Cases | Authors: Qi-Zhong Gao | Species: human | Application: IHC - Expressions of EZH2 and NOTCH3 pathway in osteosarcoma and their roles in osteosarcoma stem cells
Journal: Pol J Pathol | Authors: Guangning Yan | Species: human | Application: IHC - Neuropathological and biochemical assessments of an Alzheimer's disease patient treated with the γ-secretase inhibitor semagacestat.
Journal: Am J Neurodegener Dis | Authors: Alex E Roher | Species: human | Application: WB - KrasG12D upregulates Notch signaling to induce gallbladder tumorigenesis in mice.
Journal: Oncoscience | Authors: Wen-Cheng Chung | Species: mouse | Application: IHC - Integrated analysis reveals the dysfunction of intercellular communication and metabolic signals in dilated cardiomyopathy
Journal: Heliyon | Authors: Rui Shi | Species: mouse | Application: WB
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