RPS14 Polyclonal antibody 16683-1-AP proteintech
$149.00
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SKU
16683-1-AP
| Catalog Number | 16683-1-AP |
| Synonyms | Small ribosomal subunit protein uS11, RPS 14, ribosomal protein S14, PRO2640, EMTB |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, zebrafish, arabidopsis |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells, rat liver tissue |
| Tested Applications — Positive IHC detected in | human liver tissue, human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue, human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, arabidopsis |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10012 Product name: Recombinant human RPS14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC006784 Sequence: MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL Predict reactive species |
| Full Name | ribosomal protein S14 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC006784 |
| Gene Symbol | RPS14 |
| Gene ID (NCBI) | 6208 |
| RRID | AB_2180158 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62263 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RPS14 antibody 16683-1-AP | Download protocol |
| WB protocol for RPS14 antibody 16683-1-AP | Download protocol |
| human | IF Nucleic Acids Res Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. Authors - Fujimura Ken K View Article |
| mouse | WB Nat Commun DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death Authors - Honghao Bi View Article |
| human, mouse, rat | WB,IP,IHC,IF,ELISA Dev Cell P5CS represses tumor progression via inhibiting assembly of 48S pre-translation initiation complex. Authors - Qingqing Zhang View Article |
| Citations | 21 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631]. Protocols Product Specific Protocols IHC protocol for RPS14 antibody 16683-1-AP Download protocol WB protocol for RPS14 antibody 16683-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Rpl12 is a conserved ribophagy receptor
Journal: Nat Cell Biol | Authors: Yuting Chen | Species: human | Application: WB - Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis
Journal: Nat Commun | Authors: Daniel J Ham | Species: mouse | Application: WB - An oocyte meiotic midbody cap is required for developmental competence in mice
Journal: Nat Commun | Authors: Gyu Ik Jung | Species: mouse | Application: IF - DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death
Journal: Nat Commun | Authors: Honghao Bi | Species: mouse | Application: WB - Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
Journal: Nucleic Acids Res | Authors: Fujimura Ken K | Species: human | Application: IF - P5CS represses tumor progression via inhibiting assembly of 48S pre-translation initiation complex.
Journal: Dev Cell | Authors: Qingqing Zhang | Species: human, mouse, rat | Application: WB,IP,IHC,IF,ELISA - P5CS represses tumor progression via inhibiting assembly of 48S pre-translation initiation complex.
Journal: Dev Cell | Authors: Qingqing Zhang | Species: human, mouse, rat | Application: WB,IP,IHC,IF,ELISA - CircRNA-RBAC induces cardiac repair by promoting ribosome biogenesis and cell cycle progression in cardiomyocytes
Journal: Int J Biol Macromol | Authors: Senlin Huang | Application: WB - Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.
Journal: Free Radic Biol Med | Authors: Reiko Iida | Species: human | Application: WB - TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling.
Journal: Blood Adv | Authors: Oscar A Peña | Species: zebrafish | Application: IF - Local protein synthesis at neuromuscular synapses is required for motor functions
Journal: Cell Rep | Authors: Wen-Yo Tu | Species: human - The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis.
Journal: J Exp Bot | Authors: Ilyeong Choi | Species: Arabidopsis | Application: WB - Altered ribosomal function and protein synthesis caused by tau.
Journal: Acta Neuropathol Commun | Authors: Harrison Tudor Evans | Species: human | Application: WB - The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis.
Journal: J Exp Bot | Authors: Ilyeong Choi | Species: Arabidopsis | Application: WB - Kin17 promotes rDNA transcription, ribosomal biogenesis, and cortical lamination.
Journal: EMBO Rep | Authors: Wenbo Li | Species: mouse | Application: WB - Identification of new interactors of eIF3f by endogenous proximity-dependent biotin labelling in human muscle cells.
Journal: Sci Rep | Authors: Lionel Tintignac | Species: human | Application: WB - Differential expression of ribosomal proteins in myelodysplastic syndromes.
Journal: J Clin Pathol | Authors: Elizabeth B Rinker | Species: human | Application: IHC - Gene Expression Changes by Diallyl Trisulfide Administration in Chemically-induced Mammary Tumors in Rats.
Journal: J Cancer Prev | Authors: Eun-Ryeong Hahm | Species: rat | Application: WB - DDX41 dissolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death
Journal: bioRxiv | Authors: Honghao Bi | Species: mouse | Application: WB - An oocyte meiotic midbody cap is required for developmental competence in mice
Journal: Res Sq | Authors: Karen Schindler | Species: mouse | Application: IF - Detection of ribophagy in yeast and mammals
Journal: Biophys Rep | Authors: Miao Ye | Application: WB
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