RPS27 Polyclonal antibody 15355-1-AP proteintech
$149.00
In stock
SKU
15355-1-AP
| Catalog Number | 15355-1-AP |
| Synonyms | MPS-1, MPS1, MPS 1, Metallopan-stimulin 1, Metallopan stimulin 1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, SGC-7901 cells, Jurkat cells |
| Tested Applications — Positive IP detected in | SGC-7901 cells |
| Tested Applications — Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HepG2 cells, SGC-7901 cells, Jurkat cells |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7601 Product name: Recombinant human RPS27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC002658 Sequence: MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH Predict reactive species |
| Full Name | ribosomal protein S27 |
| Calculated Molecular Weight | 9 kDa |
| Observed Molecular Weight | 9 kDa |
| GenBank Accession Number | BC002658 |
| Gene Symbol | RPS27 |
| Gene ID (NCBI) | 6232 |
| RRID | AB_2180509 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42677 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IHC protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IP protocol for RPS27 antibody 15355-1-AP | Download protocol |
| WB protocol for RPS27 antibody 15355-1-AP | Download protocol |
| human | WB Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation Authors - Yu Fan View Article |
| human,mouse | WB Cancer Metab High-fat diet promotes prostate cancer metastasis via RPS27 Authors - Dameng Li KD Validated View Article |
| Citations | 7 |
| Dilutions | WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells.
Journal: Nat Commun | Authors: Hyun Ju Lee | Species: human | Application: WB - The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells.
Journal: FEBS Lett | Authors: Isabel Saez | Species: human | Application: WB - FKBP10 Regulates Protein Translation to Sustain Lung Cancer Growth.
Journal: Cell Rep | Authors: Giorgio Ramadori | Species: human - Defective Human SRP Induces Protein Quality Control and Triggers Stress Response
Journal: J Mol Biol | Authors: Elena B Tikhonova | Species: human | Application: WB - GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation
Journal: Brain | Authors: Yu Fan | Species: human | Application: WB - High-fat diet promotes prostate cancer metastasis via RPS27
Journal: Cancer Metab | Authors: Dameng Li KD Validated | Species: human,mouse | Application: WB - The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells.
Journal: FEBS Lett | Authors: Isabel Saez | Species: human | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
