RPS3 Recombinant monoclonal antibody 84947-4-RR proteintech
$149.00
In stock
SKU
84947-4-RR
| Catalog Number | 84947-4-RR |
| Synonyms | 242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, COLO 320 cells, NIH/3T3 cells, mouse brain tissue, human brain tissue, |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | NIH/3T3 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HEK-293 cells, COLO 320 cells, NIH/3T3 cells, mouse brain tissue, human brain tissue, |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag7346 Product name: Recombinant human RPS3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-243 aa of BC003137 Sequence: MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA Predict reactive species |
| Full Name | ribosomal protein S3 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC003137 |
| Gene Symbol | RPS3 |
| Gene ID (NCBI) | 6188 |
| RRID | AB_3743873 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | P23396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RPS3 antibody 84947-4-RR | Download protocol |
| IHC protocol for RPS3 antibody 84947-4-RR | Download protocol |
| WB protocol for RPS3 antibody 84947-4-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242504F3 |
| Conjugation | Unconjugated |
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. Protocols Product Specific Protocols IF protocol for RPS3 antibody 84947-4-RR Download protocol IHC protocol for RPS3 antibody 84947-4-RR Download protocol WB protocol for RPS3 antibody 84947-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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