CoraLite® Plus 488-conjugated RRM1 Polyclonal antibody CL488-10526 proteintech
$479.00
In stock
SKU
CL488-10526
| Catalog Number | CL488-10526 |
| Synonyms | EC:1.17.4.1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, ribonucleotide reductase M1, RIR1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat, monkey |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, monkey |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0789 Product name: Recombinant human RRM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 593-792 aa of BC006498 Sequence: IRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS Predict reactive species |
| Full Name | ribonucleotide reductase M1 |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC006498 |
| Gene Symbol | RRM1 |
| Gene ID (NCBI) | 6240 |
| RRID | AB_2918984 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Antigen Affinity Purified |
| UNIPROT ID | P23921 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 488 RRM1 antibody CL488-10526 | Download protocol |
| IF protocol for CL Plus 488 RRM1 antibody CL488-10526 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | CoraLite® Plus 488 |
Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair. What is the molecular weight of RRM1? The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 subunits in non-dividing quiescent cells (PMID: 17416930) . What is the subcellular localization of RRM1? RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953). What is the tissue expression pattern of RRM1? RRM1 is ubiquitously expressed. How is RRM1 expression regulated during the cell cycle and upon DNA damage? Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592). Protocols Product Specific Protocols FC protocol for CL Plus 488 RRM1 antibody CL488-10526 Download protocol IF protocol for CL Plus 488 RRM1 antibody CL488-10526 Download protocol Standard Protocols Click here to view our Standard Protocols
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