SH2D3C Polyclonal antibody 12882-1-AP proteintech
$149.00
In stock
SKU
12882-1-AP
| Catalog Number | 12882-1-AP |
| Synonyms | CHAT, Novel SH2 containing protein 3, NSP3, PRO34088, SH2 domain containing 3C, SH2D3C |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, human brain tissue, human placenta tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue, |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2400 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | HEK-293 cells, human brain tissue, human placenta tissue |
| Positive IP detected in | mouse brain tissue, |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3887 Product name: Recombinant human SH2D3C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 354-703 aa of BC027962 Sequence: TETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEVTSSFNPATFQSLLIPRDNRPLEVGLLRKVKELLAEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEERAALLHKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEKKLKPFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLEAARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEKFDKVLTALSHKLEPAVRSSEL Predict reactive species |
| Full Name | SH2 domain containing 3C |
| Calculated Molecular Weight | 94 kDa, 86 kDa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC027962 |
| Gene Symbol | SH2D3C |
| Gene ID (NCBI) | 10044 |
| RRID | AB_2188993 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N5H7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for SH2D3C antibody 12882-1-AP | Download protocol |
| IP protocol for SH2D3C antibody 12882-1-AP | Download protocol |
| WB protocol for SH2D3C antibody 12882-1-AP | Download protocol |
| rat | WB,IP Neuroscience Up-regulation of NSP3 by oligomeric Aβ accelerates neuronal death through Cas-independent Rap1A activation. Authors - Fujiya Gomi View Article |
| Citations | 1 |
| Dilutions | WB : 1:500-1:2400 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SH2 domain-containing protein 3C (SH2D3C, or NSP3) gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein was identified as an Eph receptor-binding protein which may be a positive regulator of TCR signaling (PMID: 10542222). SH2D3C is an adaptor protein that has been implicated in T cell activation and shown to associate with different components of the integrin signaling pathway ex vivo, moreover, its primary in vivo role of Sh2d3c in regulating B cell development and function has also been demonstrated (PMID: 20505138). SH2D3C has 6 isoforms with MW 94, 86, 77 and 55 kDa (refer to UniProt). Protocols Product Specific Protocols IHC protocol for SH2D3C antibody 12882-1-AP Download protocol IP protocol for SH2D3C antibody 12882-1-AP Download protocol WB protocol for SH2D3C antibody 12882-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Up-regulation of NSP3 by oligomeric Aβ accelerates neuronal death through Cas-independent Rap1A activation.
Journal: Neuroscience | Authors: Fujiya Gomi | Species: rat | Application: WB,IP
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