SH3D19 Polyclonal antibody 21499-1-AP proteintech
$149.00
In stock
SKU
21499-1-AP
| Catalog Number | 21499-1-AP |
| Synonyms | ADAM binding protein Eve 1, ADAM-binding protein Eve-1, EBP, EEN binding protein, EEN-binding protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HT-1080 cells, Y79 cells |
| Tested Applications — Positive IHC detected in | mouse heart tissue, human heart tissue, human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A431 cells, HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HT-1080 cells, Y79 cells |
| Positive IHC detected in | mouse heart tissue, human heart tissue, human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag15915 Product name: Recombinant human SH3D19 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 415-764 aa of BC108890 Sequence: VLVMLKQTENNYLECQKGEDTGRVHLSQMKIITPLDEHLRSRPNDPSHAQKPVDSGAPHAVVLHDFPAEQVDDLNLTSGEIVYLLEKIDTDWYRGNCRNQIGIFPANYVKVIIDIPEGGNGKRECVSSHCVKGSRCVARFEYIGEQKDELSFSEGEIIILKEYVNEEWARGEVRGRTGIFPLNFVEPVEDYPTSGANVLSTKVPLKTKKEDSGSNSQVNSLPAEWCEALHSFTAETSDDLSFKRGDRIQILERLDSDWCRGRLQDREGIFPAVFVRPCPAEAKSMLAIVPKGRKAKALYDFRGENEDELSFKAGDIITELESVDDDWMSGELMGKSGIFPKNYIQFLQIS Predict reactive species |
| Full Name | SH3 domain containing 19 |
| Calculated Molecular Weight | 790 aa, 87 kDa |
| Observed Molecular Weight | 45-47 kDa |
| GenBank Accession Number | BC108890 |
| Gene Symbol | SH3D19 |
| Gene ID (NCBI) | 152503 |
| RRID | AB_10733218 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5HYK7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SH3D19 antibody 21499-1-AP | Download protocol |
| IHC protocol for SH3D19 antibody 21499-1-AP | Download protocol |
| WB protocol for SH3D19 antibody 21499-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IHC : 1:200-1:800 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SH3D19, also known as EBP, Eve-1, encodes a multiple SH3 domain-containing protein. SH3D19 plays a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. SH3D19 is involved in the suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1(PMID: 14551139). SH3D19 is abundantly expressed in skeletal muscle and heart(PMID: 15280379). Protocols Product Specific Protocols IF protocol for SH3D19 antibody 21499-1-AP Download protocol IHC protocol for SH3D19 antibody 21499-1-AP Download protocol WB protocol for SH3D19 antibody 21499-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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