SH3PXD2A Polyclonal antibody 18976-1-AP proteintech
$149.00
In stock
SKU
18976-1-AP
| Catalog Number | 18976-1-AP |
| Synonyms | FISH, TKS5, SH3MD1, SH3 and PX domain-containing protein 2A, KIAA0418 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | SH3 and PX domains 2A |
| Calculated Molecular Weight | 125 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | NM_014631 |
| Gene Symbol | SH3PXD2A |
| Gene ID (NCBI) | 9644 |
| RRID | AB_10598319 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TCZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SH3PXD2A antibody 18976-1-AP | Download protocol |
| IHC protocol for SH3PXD2A antibody 18976-1-AP | Download protocol |
| WB protocol for SH3PXD2A antibody 18976-1-AP | Download protocol |
| human | WB,IHC,IF J Oncol Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma Authors - Koroku Kato KD Validated View Article |
| mouse | IF Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. Authors - Dong Ma View Article |
| Citations | 15 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:20-1:200 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts (PMID: 19464300). The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150 kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells (PMID: 9687503). This antibody is specific to SH3PXD2A. Protocols Product Specific Protocols IF protocol for SH3PXD2A antibody 18976-1-AP Download protocol IHC protocol for SH3PXD2A antibody 18976-1-AP Download protocol WB protocol for SH3PXD2A antibody 18976-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.
Journal: Nat Commun | Authors: Shu-Chen Liu | Species: human | Application: WB - Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.
Journal: Circ. Res. | Authors: Dong Ma | Species: mouse | Application: IF - Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration
Journal: Adv Healthc Mater | Authors: Zhao Wei | Species: human | Application: IF - IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.
Journal: Theranostics | Authors: Ge Liu - The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.
Journal: Oncotarget | Authors: Shinji Iizuka KD Validated | Species: human | Application: WB - Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma
Journal: J Oncol | Authors: Koroku Kato KD Validated | Species: human | Application: WB,IHC,IF - An oncogenic isoform of septin 9 promotes the formation of juxtanuclear invadopodia by reducing nuclear deformability
Journal: Cell Rep | Authors: Joshua Okletey | Species: human | Application: IF - Expression of non-phosphorylatable S5A-L-plastin exerts phenotypes distinct from L-plastin deficiency during podosome formation and phagocytosis
Journal: Front Cell Dev Biol | Authors: Xue Lin | Species: mouse | Application: IF - The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.
Journal: Oncotarget | Authors: Shinji Iizuka | Species: human | Application: WB - Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma
Journal: J Oncol | Authors: Koroku Kato | Species: human | Application: WB,IHC,IF - Unveiling Epithelial Plasticity Regulation in Lung Cancer: Exploring the Crosstalk Among Tks4 Scaffold Protein Partners
Journal: Mol Biol Cell | Authors: Loretta László | Species: human | Application: WB,IF - The clinicopathologic significance of Tks5 expression of peritoneal mesothelial cells in gastric cancer patients.
Journal: PLoS One | Authors: Atsushi Sugimoto | Species: human | Application: IHC - The clinicopathologic significance of Tks5 expression of peritoneal mesothelial cells in gastric cancer patients.
Journal: PLoS One | Authors: Atsushi Sugimoto | Species: human | Application: IHC - Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells.
Journal: Prostate | Authors: Burger Karen L KL | Species: human | Application: IHC - Gab1 regulates invadopodia and autocrine VEGF through SHP2/ERK1/2 in hilar cholangiocarcinoma cells
Journal: Am J Transl Res | Authors: Tingting Li | Species: human | Application: WB
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