SIRT4 Polyclonal antibody 28148-1-AP proteintech
$149.00
In stock
SKU
28148-1-AP
| Catalog Number | 28148-1-AP |
| Synonyms | EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse heart tissue, mouse liver tissue |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | mouse heart tissue, mouse liver tissue |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28003 Product name: Recombinant human SIRT4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 149-306 aa of BC109319 Sequence: HTKAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEEQVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKLNSRCGE Predict reactive species |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) |
| Calculated Molecular Weight | 314 aa, 35 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC109319 |
| Gene Symbol | SIRT4 |
| Gene ID (NCBI) | 23409 |
| RRID | AB_3742279 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6E7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SIRT4 antibody 28148-1-AP | Download protocol |
| WB protocol for SIRT4 antibody 28148-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:6000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development. Protocols Product Specific Protocols IF protocol for SIRT4 antibody 28148-1-AP Download protocol WB protocol for SIRT4 antibody 28148-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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