SKIV2L2 Recombinant monoclonal antibody 86536-1-RR proteintech
$149.00
In stock
SKU
86536-1-RR
| Catalog Number | 86536-1-RR |
| Synonyms | ATP dependent helicase SKIV2L2, ATP-dependent RNA helicase DOB1, ATP-dependent RNA helicase SKIV2L2, DOB1, EC:3.6.4.13 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, IMR-32 cells, Jurkat cells, HEK-293 cells, Neuro-2a cells, mouse testis tissue |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | HepG2 cells, IMR-32 cells, Jurkat cells, HEK-293 cells, Neuro-2a cells, mouse testis tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag3414 Product name: Recombinant human SKIV2L2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 3-307 aa of BC028604 Sequence: DAFGDELFSVFEGDSTTAAGTKKDKEKDKGKWKGPPGSADKAGKRFDGKLQSESTNNGKNKRDVDFEGTDEPIFGKKPRIEESITEDLSLADLMPRVKVQSVETVEGCTHEVALPAEEDYLPLKPRVGKAAKEYPFILDAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVI Predict reactive species |
| Full Name | superkiller viralicidic activity 2-like 2 (S. cerevisiae) |
| Calculated Molecular Weight | 1042 aa, 118 kDa |
| Observed Molecular Weight | 118 kDa |
| GenBank Accession Number | BC028604 |
| Gene Symbol | SKIV2L2 |
| Gene ID (NCBI) | 23517 |
| RRID | AB_3744952 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P42285 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SKIV2L2 antibody 86536-1-RR | Download protocol |
| WB protocol for SKIV2L2 antibody 86536-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 251430D11 |
| Conjugation | Unconjugated |
SKIV2L2 (Ski2-like RNA helicase 2, also called Mtr4) is an evolutionarily conserved 3′→5′ RNA helicase belonging to the Ski2-like subfamily of DExH-box helicases.SKIV2L2 is an essential component of the nuclear exosome targeting (NEXT) complex, where it acts as the major ATP-dependent motor that threads RNA substrates into the catalytic core of the RNA exosome for processing and degradation. Through this activity, SKIV2L2 regulates the turnover of aberrant pre-mRNAs, non-coding RNAs, and promoter-associated RNAs, thereby influencing gene expression, ribosomal RNA biogenesis, and maintenance of nuclear RNA homeostasis. It plays a role in cell differentiation and spermatogenesis. Clinically, germline mutations in SKIV2L2 have been associated with tricho-hepato-enteric syndrome, highlighting its importance in intestinal and hepatic development. (PMID: 39863862) Protocols Product Specific Protocols IF protocol for SKIV2L2 antibody 86536-1-RR Download protocol WB protocol for SKIV2L2 antibody 86536-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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