CoraLite® Plus 750-conjugated ANT1/2 Recombinant monoclonal antibody CL750-83104 proteintech
$479.00
In stock
SKU
CL750-83104
| Catalog Number | CL750-83104 |
| Synonyms | SLC25A5, 230247B1, 2F1, AAC2, ADP,ATP carrier protein 2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | WB |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | C6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells |
| Tested Applications — Positive FC (Intra) detected in | PC-3 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | C6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells |
| Positive FC (Intra) detected in | PC-3 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag11956 Product name: Recombinant human SLC25A5 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-298 aa of BC056160 Sequence: MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT Predict reactive species |
| Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 |
| Calculated Molecular Weight | 298 aa, 33 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC056160 |
| Gene Symbol | SLC25A5 |
| Gene ID (NCBI) | 292 |
| RRID | AB_3673767 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Excitation Laser | Red Laser (633 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P05141 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 750 ANT1/2 antibody CL750-83104 | Download protocol |
| WB protocol for CL Plus 750 ANT1/2 antibody CL750-83104 | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:1000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230247B1 |
| Conjugation | CoraLite® Plus 750 |
Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112) Protocols Product Specific Protocols FC protocol for CL Plus 750 ANT1/2 antibody CL750-83104 Download protocol WB protocol for CL Plus 750 ANT1/2 antibody CL750-83104 Download protocol Standard Protocols Click here to view our Standard Protocols
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