SNX17 Polyclonal antibody 10275-1-AP proteintech
$149.00
In stock
SKU
10275-1-AP
| Catalog Number | 10275-1-AP |
| Synonyms | KIAA0064, Sorting nexin-17 |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | K-562 cells, HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, mouse cerebellum tissue, RAW 264.7 cells, SK-N-SH cells, SH-SY5Y cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | K-562 cells, HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, mouse cerebellum tissue, RAW 264.7 cells, SK-N-SH cells, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0355 Product name: Recombinant human SNX17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 17-226 aa of BC002524 Sequence: GSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYA Predict reactive species |
| Full Name | sorting nexin 17 |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC002524 |
| Gene Symbol | SNX17 |
| Gene ID (NCBI) | 9784 |
| RRID | AB_2192394 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15036 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for SNX17 antibody 10275-1-AP | Download protocol |
| IP protocol for SNX17 antibody 10275-1-AP | Download protocol |
| WB protocol for SNX17 antibody 10275-1-AP | Download protocol |
| human | WB Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. Authors - Yunkai Zhu View Article |
| mouse | WB Proc Natl Acad Sci U S A LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins. Authors - Björn Schorch KD Validated View Article |
| Citations | 26 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled-coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin and may function in the intracellular trafficking of P-selectin. Protocols Product Specific Protocols IHC protocol for SNX17 antibody 10275-1-AP Download protocol IP protocol for SNX17 antibody 10275-1-AP Download protocol WB protocol for SNX17 antibody 10275-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- A phosphoinositide conversion mechanism for exit from endosomes.
Journal: Nature | Authors: Katharina Ketel KD Validated | Species: human | Application: WB - Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.
Journal: Nat Cell Biol | Authors: B?ttcher Ralph Thomas RT KD Validated | Species: mouse | Application: WB - Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
Journal: Nat Cell Biol | Authors: Kerrie E McNally | Species: human | Application: WB - Recycling of autophagosomal components from autolysosomes by the recycler complex.
Journal: Nat Cell Biol | Authors: Chuchu Zhou | Species: human | Application: WB - A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.
Journal: Nat Commun | Authors: Yunkai Zhu | Species: human | Application: WB - LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.
Journal: Proc Natl Acad Sci U S A | Authors: Björn Schorch KD Validated | Species: mouse | Application: WB - Mechanism and regulation of cargo entry into the Commander endosomal recycling pathway
Journal: Nat Commun | Authors: Rebeka Butkovič | Species: human | Application: WB,IF - A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.
Journal: Nat Commun | Authors: Yunkai Zhu | Species: human | Application: WB - SNX17 protects the heart from doxorubicin-induced cardiotoxicity by modulating LMOD2 degradation.
Journal: Pharmacol Res | Authors: Yanping Zhang | Species: rat | Application: WB - LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.
Journal: Proc Natl Acad Sci U S A | Authors: Björn Schorch | Species: mouse | Application: WB - Suppression of Membranous LRP5 Recycling, Wnt/β-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P.
Journal: Cell Rep | Authors: Fuyao Liu | Species: human | Application: WB,IF - Sorting nexin 17 increases low-density lipoprotein receptor-related protein 4 membrane expression: A novel mechanism of acetylcholine receptor aggregation in myasthenia gravis
Journal: Front Immunol | Authors: Xiaoxiao He | Species: mouse | Application: WB,IP - SNX17 knockdown improves post-ischemic angiogenesis via blocking lysosomal dependent VEGFR degradation
Journal: Life Sci | Authors: Yang Tang | Application: IF - TFEB controls retromer expression in response to nutrient availability.
Journal: J Cell Biol | Authors: Rachel Curnock | Species: human | Application: WB - The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation
Journal: EMBO Rep | Authors: Kaikai Yu | Species: human | Application: WB - SNX17 Mediates Dendritic Spine Maturation via p140Cap
Journal: Mol Neurobiol | Authors: Qiuyan Cui | Species: mouse | Application: WB - Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability.
Journal: J Mol Biol | Authors: Hui-Yuan Tseng | Species: mouse,human | Application: WB - Phosphorylation of SNX17 impedes activation of Retriever-mediated sorting
Journal: J Biol Chem | Authors: Jan Dominik Speidel | Species: mouse | Application: WB - Phosphorylation of SNX17 impedes activation of Retriever-mediated sorting
Journal: J Biol Chem | Authors: Jan Dominik Speidel | Species: mouse | Application: WB - SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling.
Journal: J Immunol | Authors: Douglas G Osborne | Species: human | Application: WB,IF - Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.
Journal: J Cell Sci | Authors: Alba Zuidema | Species: mouse | Application: WB - Sorting nexins associated with recycling complexes regulate epithelial sodium channel trafficking.
Journal: Am J Physiol Renal Physiol | Authors: Monique L Scott | Species: human, mouse, rat | Application: WB - New insights into the phosphorylation of the threonine motif of the β1 integrin cytoplasmic domain.
Journal: Life Sci Alliance | Authors: Ralph T Böttcher | Species: mouse | Application: WB - The Role of Sorting Nexin 17 in Cardiac Development.
Journal: Front Cardiovasc Med | Authors: Yufei Wu | Species: rat | Application: WB - Regulation of Cell Migration and β1 Integrin Trafficking by the Endosomal Adaptor GGA3.
Journal: Traffic | Authors: Colin D H Ratcliffe | Species: human | Application: WB,IP,IF - Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface.
Journal: Biochim Biophys Acta | Authors: Adachi Hideki H | Species: human | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
