| Catalog Number | CL594-66474 |
| Synonyms | SODM, 3A6C2, EC:1.15.1.1, IPO B, IPOB |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive IF/ICC detected in | HUVEC cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC (Intra) detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag21388 Product name: Recombinant human SOD2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-140 aa of BC016934 Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV Predict reactive species |
| Full Name | superoxide dismutase 2, mitochondrial |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC016934 |
| Gene Symbol | SOD2 |
| Gene ID (NCBI) | 6648 |
| RRID | AB_2883586 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P04179 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL594 SOD2 antibody CL594-66474 | Download protocol |
| IF protocol for CL594 SOD2 antibody CL594-66474 | Download protocol |
| human | IF Cell Death Dis Single-cell transcriptome profiles the heterogeneity of tumor cells and microenvironments for different pathological endometrial cancer and identifies specific sensitive drugs Authors - Fang Ren View Article |
| Citations | 2 |
| Dilutions | IF/ICC : 1:50-1:500 FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 3A6C2 |
| Conjugation | CoraLite®594 |
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. Protocols Product Specific Protocols FC protocol for CL594 SOD2 antibody CL594-66474 Download protocol IF protocol for CL594 SOD2 antibody CL594-66474 Download protocol Standard Protocols Click here to view our Standard Protocols
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