Flotillin 1 Polyclonal antibody 15571-1-AP proteintech
$149.00
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SKU
15571-1-AP
| Catalog Number | 15571-1-AP |
| Synonyms | FLOT1, Flotillin-1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA, IB |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7902 Product name: Recombinant human Flotillin 1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 80-427 aa of BC001146 Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA Predict reactive species |
| Full Name | flotillin 1 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC001146 |
| Gene Symbol | Flotillin 1 |
| Gene ID (NCBI) | 10211 |
| RRID | AB_2106746 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IHC protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IP protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| WB protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| human | WB Adv Sci (Weinh) Cancer Cell-Derived Large Extracellular Vesicles Promote Venous Thromboembolism by Activating NETosis Through Delivering CYBA Authors - Xiangji Li View Article |
| mouse,human | WB Theranostics Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization Authors - Yuting Lin View Article |
| mouse | WB Cell Res Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death. Authors - Xin Chen View Article |
| Bruna (Verified Customer) (06-10-2026) | worked well Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mouse samples Flotillin 1 Antibody Western Blot validation (1:1000 dilution) in mouse samples (Cat no:15571-1-AP) |
| Guorong (Verified Customer) (03-22-2022) | Good performance with a specific band of expected size and 2 bigger and weaker bands Applications: Western Blot Primary Antibody Dilution: 1:2500 Cell Tissue Type: ARPE-19 Flotillin 1 Antibody Western Blot validation (1:2500 dilution) in ARPE-19 (Cat no:15571-1-AP) |
| Citations | 74 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth. Protocols Product Specific Protocols IF protocol for Flotillin 1 antibody 15571-1-AP Download protocol IHC protocol for Flotillin 1 antibody 15571-1-AP Download protocol IP protocol for Flotillin 1 antibody 15571-1-AP Download protocol WB protocol for Flotillin 1 antibody 15571-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.
Journal: J Extracell Vesicles | Authors: Xiaohan Gao | Species: human | Application: WB - Extracellular vesicle biomarkers for complement dysfunction in schizophrenia
Journal: Brain | Authors: Ting Xue | Species: human | Application: WB - Lysosome-Featured Cell Aggregate-Released Extracellular Vesicles Regulate Iron Homeostasis and Alleviate Post-Irradiation Endothelial Ferroptosis for Mandibular Regeneration
Journal: Adv Sci (Weinh) | Authors: Yuan-Yuan Li | Species: human | Application: WB - Cancer Cell-Derived Large Extracellular Vesicles Promote Venous Thromboembolism by Activating NETosis Through Delivering CYBA
Journal: Adv Sci (Weinh) | Authors: Xiangji Li | Species: human | Application: WB - Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization
Journal: Theranostics | Authors: Yuting Lin | Species: mouse,human | Application: WB - Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.
Journal: Cell Res | Authors: Xin Chen | Species: mouse | Application: WB - Mesenchymal Stem Cell-Derived Extracellular Vesicles Ameliorate Temporomandibular Joint Osteoarthritis by Suppressing Osteoclast Activity Via let-7a-5p/Integrin β3 Axis
Journal: ACS Nano | Authors: Zhihua Yang - The fatty acid receptor CD36 promotes macrophage infiltration via p110γ signaling to stimulate metastasis
Journal: J Adv Res | Authors: Hong Qin | Species: mouse,human | Application: WB,IF - Mesenchymal stem cells, as glioma exosomal immunosuppressive signal multipliers, enhance MDSCs immunosuppressive activity through the miR-21/SP1/DNMT1 positive feedback loop
Journal: J Nanobiotechnology | Authors: Wei Qiu | Species: human | Application: WB - Microenvironment-induced CREPT expression by cancer-derived small extracellular vesicles primes field cancerization
Journal: Theranostics | Authors: Yuting Lin | Species: mouse,human | Application: WB - SDC1-TGM2-FLOT1-BHMT complex determines radiosensitivity of glioblastoma by influencing the fusion of autophagosomes with lysosomes
Journal: Theranostics | Authors: Liang Zeng | Species: human | Application: WB,CoIP - Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma Therapy.
Journal: Adv Sci (Weinh) | Authors: Boyan Li | Species: human | Application: WB - Lysosome-Featured Cell Aggregate-Released Extracellular Vesicles Regulate Iron Homeostasis and Alleviate Post-Irradiation Endothelial Ferroptosis for Mandibular Regeneration
Journal: Adv Sci (Weinh) | Authors: Yuan-Yuan Li | Species: human | Application: WB - Cancer Cell-Derived Large Extracellular Vesicles Promote Venous Thromboembolism by Activating NETosis Through Delivering CYBA
Journal: Adv Sci (Weinh) | Authors: Xiangji Li | Species: human | Application: WB - Enhanced Protein Synthesis and Hippocampus-Dependent Memory via Inhibition of YTHDF2-Mediated m(6)A mRNA Degradation.
Journal: Adv Sci (Weinh) | Authors: Kuan Li | Species: mouse | Application: WB - NPM1 Mediates mRNA Sorting into Extracellular Vesicles via Specific RNA Motif Binding and Phase Separation.
Journal: Adv Sci (Weinh) | Authors: Kaixiang Zhang | Species: human | Application: WB - Endocytic Control of Cell-Autonomous and Non-Cell-Autonomous Functions of p53.
Journal: Adv Sci (Weinh) | Authors: Roberta Cacciatore | Species: human | Application: IB - A combined "eat me/don't eat me" strategy based on exosome for acute liver injury treatment
Journal: Cell Rep Med | Authors: Wei Du - Amyloid futures in the expanding pathology of brain aging and dementia
Journal: Alzheimers Dement | Authors: Max A Thorwald | Species: human | Application: WB - Extracellular vesicle biomarkers for complement dysfunction in schizophrenia
Journal: Brain | Authors: Ting Xue | Species: human | Application: WB - Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats
Journal: Cell Death Dis | Authors: Yajie Wang | Species: rat | Application: WB - FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling
Journal: Int J Biol Sci | Authors: Ran Wang | Species: mouse,human | Application: WB,IHC - EVs Containing Host Restriction Factor IFITM3 Inhibited ZIKV Infection of Fetuses in Pregnant Mice through Trans-placenta Delivery.
Journal: Mol Ther | Authors: Xue Zou | Species: human | Application: WB - Lappaconitine hydrobromide prevents NLRP3-GSDMD-mediated pyroptosis to alleviate sepsis-induced acute lung injury by enhancing NR1H3.
Journal: J Pharm Anal | Authors: Yuejia Lan | Species: human | Application: WB - Structure of the flotillin complex in a native membrane environment
Journal: Proc Natl Acad Sci U S A | Authors: Ziao Fu | Species: human | Application: WB - Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression
Journal: Dev Cell | Authors: Cheng Zhang - Apoptotic extracellular vesicles from peripancreatic adipose-derived mesenchymal stem cells ameliorate severe acute pancreatitis through the transcription factor EB-mediated autophagy-lysosomal pathway: an experimental study.
Journal: Int J Surg | Authors: Ao Wang | Species: rat | Application: WB - Icaritin ameliorates extracellular microparticles-induced inflammatory pre-metastatic niche via modulating the cGAS-STING signaling.
Journal: Phytother Res | Authors: Shumei Tu | Species: mouse | Application: WB - Recruitment of DNA to tumor-derived microvesicles.
Journal: Cell Rep | Authors: James W Clancy | Species: human | Application: WB - Flotillin-1 promotes the progression of hepatocellular carcinoma by activating TFE3-mediated Golgi stress response via inhibition of mTORC1/2.
Journal: World J Gastroenterol | Authors: Liang Zhang | Species: human | Application: WB,CoIP - Bone marrow macrophage-derived exosomal miR-143-5p contributes to insulin resistance in hepatocytes by repressing MKP5.
Journal: Cell Prolif | Authors: Linfang Li | Species: mouse | Application: WB - Identification of proteins in semen-derived extracellular vesicles that bind to Tat and NF-κB and that may impair HIV replication
Journal: Sci Signal | Authors: Bryson C Okeoma - Shenmai injection maintains blood-brain barrier integrity following focal cerebral ischemia via modulating the expression and trafficking of occludin in lipid rafts.
Journal: J Ethnopharmacol | Authors: Huaming Xu | Species: rat | Application: WB,IP - Chemical proteomics reveals Nε-fatty-acylation of septins by Rho Inactivation Domain (RID) of the Vibrio MARTX toxin to alter septin localization and organization
Journal: Mol Cell Proteomics | Authors: Yaxin Xu | Species: human | Application: WB - Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins
Journal: Mol Cell Proteomics | Authors: Fatema Akter | Species: human | Application: WB - Method Evaluation of Liquid Biopsy Proteomics for Limited Plasma Volume.
Journal: Mol Cell Proteomics | Authors: Ryo Takahashi | Species: rat | Application: WB - Mechanisms of dihydromyricetin against hepatocellular carcinoma elucidated by network pharmacology combined with experimental validation
Journal: Pharm Biol | Authors: Shuo Zhang | Species: human | Application: WB - Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease
Journal: Cells | Authors: Ahmed Elsherbini | Species: mouse | Application: WB - Exosomes Induce Fibroblast Differentiation into Cancer-associated Fibroblasts through TGFβ Signaling.
Journal: Mol Cancer Res | Authors: Cassandra R Goulet | Species: human | Application: WB - Distinct populations of lung capillary endothelial cells and their functional significance.
Journal: Commun Biol | Authors: Joel James | Species: rat - A pore-forming protein drives macropinocytosis to facilitate toad water maintaining.
Journal: Commun Biol | Authors: Zhong Zhao | Application: WB - EIF4A3 acts on the PI3K-AKT-ERK1/2-P70S6K pathway through FLOT1 to influence the development of lung adenocarcinoma
Journal: Mol Cancer Res | Authors: Wenhao Yu | Species: human | Application: WB,IP - GBA Mutations Influence the Release and Pathological Effects of Small Extracellular Vesicles from Fibroblasts of Patients with Parkinson's Disease.
Journal: Int J Mol Sci | Authors: Silvia Cerri | Species: human | Application: WB - TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.
Journal: Int J Mol Sci | Authors: Tao Zhang | Species: human | Application: WB - Mechanism of Tau Protein Incorporation into Exosomes via Cooperative Recognition of KFERQ-like Motifs by LAMP2A and HSP70
Journal: Neurochem Int | Authors: Shan Xu | Species: human | Application: WB - Exosomal Src from hypoxic vascular smooth muscle cells exacerbates ischemic brain injury by promoting M1 microglial polarization
Journal: Neurochem Int | Authors: Xiaoting Zhang | Species: human | Application: WB - Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection
Journal: mBio | Authors: Guorong Sun | Species: human | Application: WB - From the Cover: Tetrachlorobenzoquinone Exerts Neurological Proinflammatory Activity by Promoting HMGB1 Release, Which Induces TLR4 Clustering within the Lipid Raft.
Journal: Toxicol Sci | Authors: Juanli Fu | Species: rat | Application: WB - Synergistic chemoimmunotherapy using pirarubicin-loaded tumor-derived extracellular vesicles for triple-negative breast cancer (TNBC).
Journal: Eur J Pharm Sci | Authors: Quanwu Sun | Species: mouse | Application: WB - Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia.
Journal: Sci Rep | Authors: Lisa Levine | Species: human | Application: WB - Dampening of ISGylation of RIG-I by ADAP regulates type I interferon response of macrophages to RNA virus infection
Journal: PLoS Pathog | Authors: Yan Wang | Species: mouse | Application: WB - Lost region in amyloid precursor protein (APP) through TALEN-mediated genome editing alters mitochondrial morphology.
Journal: Sci Rep | Authors: Yajie Wang | Species: human | Application: WB - Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.
Journal: Sci Rep | Authors: Jie Liu | Species: human | Application: WB,IP - Flotillin-1 is a prognostic biomarker for glioblastoma and promotes cancer development through enhancing invasion and altering tumour microenvironment
Journal: J Cell Mol Med | Authors: Ran Wang | Species: human | Application: WB,IHC - Pore-forming protein βγ-CAT promptly responses to fasting with capacity to deliver macromolecular nutrients
Journal: FASEB J | Authors: Zhi-Hong Shi | Species: human | Application: WB - Exosomes Derived From Bone Marrow Macrophages of HFD-Fed Mice Regulating AKT/GSK Pathway in Hepatocytes via miR-222-5p.
Journal: FASEB J | Authors: Huiyan Zuo | Species: mouse | Application: WB - Identification and characterization of the lamprey Flotillin-1 gene with a role in cell adhesion.
Journal: Fish Shellfish Immunol | Authors: Rong Xu | Species: human | Application: WB,IHC,IF - Dietary anthocyanidin pelargonidin activates G protein-coupled receptor 35.
Journal: FEBS J | Authors: Fumie Nakashima | Species: human | Application: WB - Acid sphingomyelinase recruits palmitoylated CD36 to membrane rafts and enhances lipid uptake
Journal: J Biol Chem | Authors: Meng Ding | Species: human | Application: WB - NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.
Journal: RNA Biol | Authors: Xiwen Li | Species: human | Application: WB - Exosomal PKM2 from AML cells reprograms endothelial metabolism to promote angiogenesis and chemoresistance.
Journal: Funct Integr Genomics | Authors: Xiaoting Wang - Neuronal Exosomes Secreted under Oxygen-Glucose Deprivation/Reperfusion Presenting Differentially Expressed miRNAs and Affecting Neuronal Survival and Neurite Outgrowth.
Journal: Neuromolecular Med | Authors: Chien-Sung Chiang | Species: rat | Application: WB - Acylation of MLKL Impacts Its Function in Necroptosis
Journal: ACS Chem Biol | Authors: Apoorva J Pradhan - TNFα triggers release of extracellular vesicles containing TNFR1 and TRADD, which can modulate TNFα responses of the parental cells.
Journal: Arch Biochem Biophys | Authors: Miwa Sohda | Species: human | Application: WB - A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers.
Journal: Exp Cell Res | Authors: Wei Zhou | Species: human | Application: WB - Exosomal tRF-Leu-AAG-001 derived from mast cell as a potential non-invasive diagnostic biomarker for endometriosis.
Journal: BMC Womens Health | Authors: Yingxue Li | Species: human | Application: WB - In vivo reduction of striatal D1R by RNA interference alters expression of D1R signaling-related proteins and enhances methamphetamine addiction in male rats.
Journal: Brain Struct Funct | Authors: Alison D Kreisler | Species: rat | Application: WB - Circulating Exosomal miRNAs as Novel Biomarkers for Stable Coronary Artery Disease.
Journal: Biomed Res Int | Authors: Ping Zhang | Species: human | Application: WB - Streamlined Analysis of Maternal Plasma Indicates Small Extracellular Vesicles are Significantly Elevated in Early-Onset Preeclampsia
Journal: Reprod Sci | Authors: Scout Bowman-Gibson | Species: human | Application: WB - Structural and Functional Basis of G Protein-Coupled Receptor 35 Activation by Pyrroloquinoline Quinone.
Journal: Biochemistry | Authors: Harumi Ando | Species: human | Application: WB - Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.
Journal: Transplant Direct | Authors: Robert W Hu | Species: human | Application: WB - Discovery of new receptors regulating LH and FSH secretion by bovine gonadotrophs to explore a new paradigm for mechanisms regulating reproduction.
Journal: J Reprod Dev | Authors: Hiroya Kadokawa | Application: IF - Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.
Journal: Cardiol Young | Authors: Li Zhang | Species: human | Application: WB - Acylation of MLKL impacts its function in necroptosis
Journal: bioRxiv | Authors: Apoorva J Pradhan | Species: human | Application: WB - Inducing oxidative stress by targeting the cholesterol biosynthesis pathway augments the efficacy of bortezomib in multiple myeloma.
Journal: Free Radic Biol Med | Authors: Chunfan Li | Species: human | Application: WB - Investigation of plasma-derived extracellular vesicles in hypertrophic cardiomyopathy patients.
Journal: iScience | Authors: Alessandra Stefania Rizzuto | Species: human | Application: WB
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