SPC25 Recombinant monoclonal antibody 87323-1-RR proteintech
$149.00
In stock
SKU
87323-1-RR
| Catalog Number | 87323-1-RR |
| Synonyms | AD024, hSpc25, Kinetochore protein Spc25, SPBC25 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, SKOV-3 cells, THP-1 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, SKOV-3 cells, THP-1 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag23985 Product name: Recombinant human SPC25 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC022255 Sequence: MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN Predict reactive species |
| Full Name | SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC022255 |
| Gene Symbol | SPC25 |
| Gene ID (NCBI) | 57405 |
| RRID | AB_3745470 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HBM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for SPC25 antibody 87323-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 252476G7 |
| Conjugation | Unconjugated |
Spindle Pole Body Component 25 (SPC25), a core subunit of the NDC80 kinetochore complex, is essential for accurate chromosome alignment and segregation during mitosis . By linking microtubules to kinetochores, SPC25 ensures proper attachment and tension generation, critical for the maintenance of genomic stability . SPC25 is largely found in the cytoplasm and nucleus. The NDC80 complex, which is involved in kinetochore-microtubule interactions and spindle assembly checkpoint activity, is composed of four subunits: SPC24, SPC25, NDC80, and NUF2. (PMID: 40587622 ,PMID: 40400636) Protocols Product Specific Protocols WB protocol for SPC25 antibody 87323-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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