SPRED2 Polyclonal antibody 24091-1-AP proteintech
$149.00
In stock
SKU
24091-1-AP
| Catalog Number | 24091-1-AP |
| Synonyms | Sprouty-related, EVH1 domain-containing protein 2, Spred-2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | PC-12 cells, MCF-7 cells, mouse testis tissue, mouse brain tissue, rat brain tissue, T-47D cells, rat testis tissue |
| Tested Applications — Positive IHC detected in | rat testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | PC-12 cells, MCF-7 cells, mouse testis tissue, mouse brain tissue, rat brain tissue, T-47D cells, rat testis tissue |
| Positive IHC detected in | rat testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag21359 Product name: Recombinant human SPRED2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 122-214 aa of BC136334 Sequence: LIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEEI Predict reactive species |
| Full Name | sprouty-related, EVH1 domain containing 2 |
| Calculated Molecular Weight | 418 aa, 48 kDa |
| Observed Molecular Weight | 40-48 kDa |
| GenBank Accession Number | BC136334 |
| Gene Symbol | SPRED2 |
| Gene ID (NCBI) | 200734 |
| RRID | AB_2879429 |
| Conjugate | Unconjugated |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q7Z698 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SPRED2 antibody 24091-1-AP | Download protocol |
| IHC protocol for SPRED2 antibody 24091-1-AP | Download protocol |
| WB protocol for SPRED2 antibody 24091-1-AP | Download protocol |
| human | WB Int J Mol Sci SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells Authors - Tong Gao View Article |
| mouse | WB J Mol Neurosci TRIM25 Suppresses Hippocampal Neuronal Autophagy under High Glucose via Ubiquitination and Degradation of SIX2. Authors - Li Chen KD Validated View Article |
| human,mouse | WB,IHC Int J Mol Sci SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular Carcinoma Cells and Normal Hepatocytes Authors - Tianyi Wang KO Validated View Article |
| Citations | 8 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SPRED2 is a member of the SPRED family of proteins that modulate GFR signaling by inhibiting the Ras-MAPK cascade (PMID: 17094949). SPRED family members are characterized by an N-terminal Enabled/VASP homology 1 domain (EVH1), a central c-Kit binding domain (KBD) and a C-terminal Sprouty-related domain (SPR) (PMID: 17691106). Three mammalian SPREDs (SPRED1-3) have been described. In adult tissues, SPRED2 is expressed ubiquitously, but most highly expressed in glandular epithelia (PMID: 15580519; 17691106). Reduced expression level of SPRED2 has been reported in human hepatocellular carcinoma (HCC), suggesting SPRED2 may play a role in tumorgenesis (PMID: 16652141). Gene disruption of SPRED2 causes dwarfism (PMID: 15946934). Protocols Product Specific Protocols IF protocol for SPRED2 antibody 24091-1-AP Download protocol IHC protocol for SPRED2 antibody 24091-1-AP Download protocol WB protocol for SPRED2 antibody 24091-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Expression of Spred2 in the urothelial tumorigenesis of the urinary bladder
Journal: PLoS One | Authors: Shinsuke Oda | Species: human | Application: IHC - Inhibition of Spred/Sprouty Expression in the Skin of a Contact Dermatitis-Like Model.
Journal: Biol Pharm Bull | Authors: Hiroyasu Sakai | Species: mouse | Application: WB - SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular Carcinoma Cells and Normal Hepatocytes
Journal: Int J Mol Sci | Authors: Tianyi Wang KO Validated | Species: human,mouse | Application: WB,IHC - SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells
Journal: Int J Mol Sci | Authors: Tong Gao | Species: human | Application: WB - microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2
Journal: Histol Histopathol | Authors: Liang Zhu | Species: mouse | Application: IF - TRIM25 Suppresses Hippocampal Neuronal Autophagy under High Glucose via Ubiquitination and Degradation of SIX2.
Journal: J Mol Neurosci | Authors: Li Chen KD Validated | Species: mouse | Application: WB - microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2
Journal: Histol Histopathol | Authors: Liang Zhu | Species: mouse | Application: IF - Inhibition of Spred/Sprouty Expression in the Skin of a Contact Dermatitis-Like Model.
Journal: Biol Pharm Bull | Authors: Hiroyasu Sakai | Species: mouse | Application: WB
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