| Catalog Number | 68548-1-Ig |
| Synonyms | SRM, 2F4E10, EC:2.5.1.16, PAPT, SPDSY |
| Host | Mouse |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Tested Applications — Positive IF/ICC detected in | RAW 264.7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | RAW 264.7 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag33919 Product name: Recombinant human SRM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-302 aa of BC000309 Sequence: MEPGPDGPAASGPAAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGMAIGYSSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGVLCCQGECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYNSDVHRAAFVLPEFARKALNDVS Predict reactive species |
| Full Name | spermidine synthase |
| Calculated Molecular Weight | 302 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC000309 |
| Gene Symbol | Spermidine synthase |
| Gene ID (NCBI) | 6723 |
| RRID | AB_3085253 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P19623 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Spermidine synthase antibody 68548-1-Ig | Download protocol |
| WB protocol for Spermidine synthase antibody 68548-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:200-1:800 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 2F4E10 |
| Conjugation | Unconjugated |
Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111). Protocols Product Specific Protocols IF protocol for Spermidine synthase antibody 68548-1-Ig Download protocol WB protocol for Spermidine synthase antibody 68548-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols