SRRT Polyclonal antibody 20353-1-AP proteintech
$149.00
In stock
SKU
20353-1-AP
| Catalog Number | 20353-1-AP |
| Synonyms | ARS2, Arsenite resistance protein 2, Arsenite-resistance protein 2, ASR2, serrate |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells |
| Tested Applications — Positive IP detected in | Jurkat cells |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14196 Product name: Recombinant human SRRT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 527-876 aa of BC000082 Sequence: LIHTLDDRTQLWASEPGTPPLPTSLPSQNPILKNITDYLIEEVSAEEEELLGSSGGAPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEYPNEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKFVTSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQPPGPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGAGAVRPAVPTGGPPYPHAPYGAGRGNYDAFRGQGGYPGKPRNRMVRGDPRAIVEYRDLDAPDDVDFF Predict reactive species |
| Full Name | serrate RNA effector molecule homolog (Arabidopsis) |
| Calculated Molecular Weight | 876 aa, 101 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC000082 |
| Gene Symbol | SRRT |
| Gene ID (NCBI) | 51593 |
| RRID | AB_3085603 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for SRRT antibody 20353-1-AP | Download protocol |
| IP protocol for SRRT antibody 20353-1-AP | Download protocol |
| WB protocol for SRRT antibody 20353-1-AP | Download protocol |
| human | WB,IHC Oncol Rep SRRT promotes prostate cancer progression and serves as a prognostic biomarker through STAT3 pathway activation. Authors - Lingxiang Lu KD Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880). Protocols Product Specific Protocols IF protocol for SRRT antibody 20353-1-AP Download protocol IP protocol for SRRT antibody 20353-1-AP Download protocol WB protocol for SRRT antibody 20353-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- SRRT promotes prostate cancer progression and serves as a prognostic biomarker through STAT3 pathway activation.
Journal: Oncol Rep | Authors: Lingxiang Lu KD Validated | Species: human | Application: WB,IHC - A genome-wide functional analysis of conserved intronic regions reveals essential roles for speckle-associated retained introns.
Journal: Cell Rep | Authors: Shaghayegh Farhangmehr
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