ST6GALNAC6 Monoclonal antibody 66209-1-Ig proteintech
$149.00
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SKU
66209-1-Ig
| Catalog Number | 66209-1-Ig |
| Synonyms | Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6, EC:2.4.99.-, GalNAc alpha-2,6-sialyltransferase VI, hST6GalNAc VI, RP11 203J24.3 |
| Host | Mouse |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | COLO 320 cells |
| Tested Applications — Positive IHC detected in | mouse colon tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human colon cancer tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | mouse colon tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8358 Product name: Recombinant human ST6GALNAC6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 67-333 aa of BC007802 Sequence: VFHYGSLRGRSRRPVNLKKWSITDGYVPILGNKTLPSRCHQCVIVSSSSHLLGTKLGPEIERAECTIRMNDAPTTGYSADVGNKTTYRVVAHSSVFRVLRRPQEFVNRTPETVFIFWGPPSKMQKPQGSLVRVIQRAGLVFPNMEAYAVSPGRMRQFDDLFRGETGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPNYCSQRPRLQRMPYHYYEPKGPDECVTYIQNEHSRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT Predict reactive species |
| Full Name | ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 |
| Calculated Molecular Weight | 333aa,38 kDa; 374aa,41 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC007802 |
| Gene Symbol | ST6GALNAC6 |
| Gene ID (NCBI) | 30815 |
| RRID | AB_2881600 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q969X2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
| IHC protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
| WB protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
| human | IHC Cell Death Dis Deficient extravillous trophoblast invasion caused by impaired sialylation-Siglec-7 interaction contributes to recurrent pregnancy loss. Authors - Linyu Zhang View Article |
| Citations | 1 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1A7B7 |
| Conjugation | Unconjugated |
ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon. Protocols Product Specific Protocols IF protocol for ST6GALNAC6 antibody 66209-1-Ig Download protocol IHC protocol for ST6GALNAC6 antibody 66209-1-Ig Download protocol WB protocol for ST6GALNAC6 antibody 66209-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Deficient extravillous trophoblast invasion caused by impaired sialylation-Siglec-7 interaction contributes to recurrent pregnancy loss.
Journal: Cell Death Dis | Authors: Linyu Zhang | Species: human | Application: IHC
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