Thrombospondin 1 Polyclonal antibody 18304-1-AP proteintech
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SKU
18304-1-AP
| Catalog Number | 18304-1-AP |
| Synonyms | THBS1, Thrombospondin-1, Glycoprotein G, THBS, THBS 1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, pig, monkey |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, CoIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human peripheral blood platelets, A431 cells, C6 cells, HeLa cells, HUVEC cells, NIH/3T3 cells, RAW 264.7 cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | human peripheral blood platelets, A431 cells, C6 cells, HeLa cells, HUVEC cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human lung cancer tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | thrombospondin 1 |
| Calculated Molecular Weight | 129 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC028145 |
| Gene Symbol | Thrombospondin 1 |
| Gene ID (NCBI) | 7057 |
| RRID | AB_2201959 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07996 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
| WB protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
| human | WB,IHC Theranostics FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis Authors - Siwei Pan View Article |
| mouse | IF Nat Commun Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction. Authors - Juan Tang View Article |
| rat | IF Nat Commun Hydrogel with cell-cell adhesion cues enhances neural regeneration. Authors - Xiaoxuan Tang View Article |
| Emma (Verified Customer) (03-15-2022) | A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Prostate |
| Citations | 83 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa. Protocols Product Specific Protocols IHC protocol for Thrombospondin 1 antibody 18304-1-AP Download protocol WB protocol for Thrombospondin 1 antibody 18304-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.
Journal: Cancer Cell | Authors: Yansen Xiao | Species: human | Application: WB - Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis.
Journal: Bone Res | Authors: Pengqiang Lou | Species: human | Application: IHC,IF - The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.
Journal: Nat Commun | Authors: Henry Nording | Species: human | Application: WB - Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction.
Journal: Nat Commun | Authors: Juan Tang | Species: mouse | Application: IF - Hydrogel with cell-cell adhesion cues enhances neural regeneration.
Journal: Nat Commun | Authors: Xiaoxuan Tang | Species: rat | Application: IF - FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis
Journal: Theranostics | Authors: Siwei Pan | Species: human | Application: WB,IHC - Engrailed-1 inactivation leads to scarless skin wound healing through extracellular matrix remodeling
Journal: Genes Dis | Authors: Ailing Hao - Renal IGFBP6 Interacts With THBS1 to Drive Renal Cellular Senescence and Fibrosis.
Journal: Adv Sci (Weinh) | Authors: Ju-tao Yu | Species: human | Application: WB,CoIP - NAALAD2 mutations disrupt the fate of photoreceptor cells and retinal pigment epithelial cells during early retinal development
Journal: Pharmacol Res | Authors: Yuanjie Qian | Species: mouse | Application: IF - Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner
Journal: Cancer Lett | Authors: Juntao Li | Species: human | Application: IHC - THBS1 in macrophage-derived exosomes exacerbates cerebral ischemia-reperfusion injury by inducing ferroptosis in endothelial cells
Journal: J Neuroinflammation | Authors: Chang Liu | Species: mouse,human | Application: WB,IF,CoIP - Gallic acid: A promising anti-non-small cell lung cancer compound targeting early growth response protein-1 for apoptosis and ferroptosis.
Journal: Phytomedicine | Authors: Bin-Bin Li | Species: human | Application: WB - Axitinib promotes stemness and vasculogenic mimicry in triple-negative breast cancer by disrupting THBS1-CD47 axis-mediated endothelial-tumor cell communication.
Journal: Apoptosis | Authors: Lei Lei - CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis
Journal: Arterioscler Thromb Vasc Biol | Authors: Bhupesh Singla | Species: mouse,human | Application: WB,IF - Polystyrene microplastics exposure reduces meat quality and disturbs skeletal muscle angiogenesis via thrombospondin 1
Journal: Food Res Int | Authors: Yun Yang | Species: pig | Application: WB,IF - Thrombospondin-1 modulation by Bifidobacterium spp. mitigates lung damage in an acute lung injury mouse model
Journal: Microbiol Res | Authors: Yumin Kim | Species: mouse | Application: IF - Investigation of a UPR-Related Gene Signature Identifies the Pro-Fibrotic Effects of Thrombospondin-1 by Activating CD47/ROS/Endoplasmic Reticulum Stress Pathway in Lung Fibroblasts
Journal: Antioxidants (Basel) | Authors: Jun-Hui Zhan | Species: mouse | Application: WB,IHC - Human induced pluripotent stem cell-derived cardiomyocytes improve recovery from myocardial infarction in non-human primates.
Journal: Stem Cell Res Ther | Authors: Xumin Guan | Species: monkey | Application: WB - THBS2-producing matrix CAFs promote colorectal cancer progression and link to poor prognosis via the CD47-MAPK axis
Journal: Cell Rep | Authors: Zaoqu Liu | Species: human | Application: WB - Senescent BMSC-Derived Thbs1 Drives Inflammaging and Impairs Bone Regeneration by Suppressing PINK1/Parkin-Mediated Mitophagy in Macrophages.
Journal: Aging Cell | Authors: Yifeng Xing | Species: rat | Application: WB - Exosomes derived from menstrual blood stromal cells ameliorated premature ovarian insufficiency and granulosa cell apoptosis by regulating SMAD3/AKT/MDM2/P53 pathway via delivery of thrombospondin-1
Journal: Biomed Pharmacother | Authors: Aixin Song | Species: rat,human | Application: WB,IF - Clu(+) transitional alveolar epithelial cells promote lung injury repair and fibrosis progression.
Journal: Br J Pharmacol | Authors: Yuquan Wang | Species: mouse | Application: WB - Hypoxia Activated Nitric Oxide Donor Compounds for the Prevention and Treatment of Myocardial Hypoxia-Induced Injury
Journal: J Med Chem | Authors: Wanxiang Yang | Species: rat | Application: WB - Discovery of Efficient Hypoxia-Targeted NO Donor Compounds to Alleviate Hypoxia Cardiac Disease
Journal: J Med Chem | Authors: Wanxiang Yang | Species: human | Application: WB - HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.
Journal: Front Immunol | Authors: Mei-Ling Dong | Application: WB - Exploration and validation of signature genes and immune associations in septic cardiomyopathy
Journal: Clin Exp Hypertens | Authors: Zhengbo Zhao | Species: mouse | Application: WB - ADAMTS-7 Increase Contributes to Worsened Neurological Outcome in Mice on High Fat Diet.
Journal: CNS Neurosci Ther | Authors: Yang Xu | Species: mouse | Application: WB - Integrated transcriptomic and proteomic profiling reveals the key molecular signatures of brain endothelial reperfusion injury
Journal: CNS Neurosci Ther | Authors: Yabin Ji | Species: mouse | Application: WB,IF - Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos
Journal: Cell Biosci | Authors: Qianqi Zhu | Species: human | Application: IF - PA2G4 in CAFs promotes biochemical recurrence of prostate cancer via H3K18la.
Journal: Cell Biosci | Authors: Shiyu Ji | Species: human | Application: IF - LSKL mitigates dehydroepiandrosterone-induced apoptosis and oxidative stress by THBS1/PI3K/AKT pathway in rat granulosa cells.
Journal: Life Sci | Authors: Lu Zhang | Species: rat | Application: WB - Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay.
Journal: Oncotarget | Authors: Kun Jia | Species: human | Application: IHC - Ntsr1 contributes to pulmonary hypertension by enhancing endoplasmic reticulum stress via JAK2-STAT3-Thbs1 signaling
Journal: Transl Res | Authors: Zhi-Xing Wei | Species: rat | Application: WB,IF - Hybrids of carbonic anhydrase and cyclooxygenase inhibitors attenuate cardiac hypoxic inflammatory injuries
Journal: Eur J Pharmacol | Authors: Wen Zhou | Species: rat | Application: WB - Yth m6A RNA-Binding Protein 1 Regulates Osteogenesis of MC3T3-E1 Cells under Hypoxia via Translational Control of Thrombospondin-1
Journal: Int J Mol Sci | Authors: Diwen Shi | Species: mouse | Application: WB,IF - Picrorhiza scrophulariiflora ethanol extract ameliorates CCl(4)-induced acute liver injury by blocking the TSP1/TNF/NF-κB axis.
Journal: Int Immunopharmacol | Authors: Menghao Li | Species: mouse,human | Application: WB - Elevated Levels of Active GSK3β in the Blood of Patients with Myotonic Dystrophy Type 1 Correlate with Muscle Weakness.
Journal: Int J Mol Sci | Authors: Katherine Jennings | Species: human | Application: WB - Picrorhiza scrophulariiflora ethanol extract ameliorates CCl(4)-induced acute liver injury by blocking the TSP1/TNF/NF-κB axis.
Journal: Int Immunopharmacol | Authors: Menghao Li | Species: mouse,human | Application: WB - Identification of driving genes of recurrent miscarriage based on transcriptome sequencing and immunoinfiltration analysis
Journal: Int Immunopharmacol | Authors: Yijun Yang | Species: human | Application: IF - CGRP/TSP1 Signaling Dampens Corneal Inflammation and Fibrosis by Targeting A2M During Corneal Stromal Wound Healing.
Journal: Invest Ophthalmol Vis Sci | Authors: Hongqi Ge | Species: mouse | Application: IF - Knockdown of KLF6 ameliorates myocardial infarction by regulating autophagy via transcriptional regulation of PTTG1
Journal: Am J Physiol Cell Physiol | Authors: Yixin Chen | Species: mouse | Application: WB - Thrombospondin-1 Production Regulates the Inflammatory Cytokine Secretion in THP-1 Cells Through NF-κB Signaling Pathway.
Journal: Inflammation | Authors: Tian Xing | Species: human | Application: WB - Entrectinib can induce nerve cell damage by inhibiting PI3K-AKT and TGF-β signaling pathways
Journal: Front Pharmacol | Authors: Qingshan Tang | Species: human | Application: WB - Thrombospondin-1 Production Regulates the Inflammatory Cytokine Secretion in THP-1 Cells Through NF-κB Signaling Pathway.
Journal: Inflammation | Authors: Tian Xing | Species: human | Application: WB - Stem cell therapy targets THBS1 to reverse endometrial fibrosis.
Journal: Front Cell Dev Biol | Authors: Xiaochuan Yu | Species: human | Application: WB - Design and discovery of novel cyclic peptides as TSP1-CD36 interaction inhibitors for intestinal fibrosis treatment
Journal: Bioorg Chem | Authors: Wen Zhang | Species: rat | Application: WB,IF - Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.
Journal: Mol Med Rep | Authors: Jin Zhao | Species: mouse | Application: WB,IHC - Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.
Journal: Transl Oncol | Authors: Xinlu Liu | Species: human | Application: WB,IHC - Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.
Journal: Transl Oncol | Authors: Xinlu Liu | Species: human | Application: WB,IHC - Circ_0047339 promotes the activation of fibroblasts and affects the development of urethral stricture by targeting the miR-4691-5p/TSP-1 axis
Journal: Sci Rep | Authors: Ke Ding | Species: human | Application: WB - Activation of SIRT3 by Tanshinone IIA ameliorates renal fibrosis by suppressing the TGF-β/TSP-1 pathway and attenuating oxidative stress
Journal: Cell Signal | Authors: Yifeng Fan | Species: mouse,human | Application: WB,IHC - Deletion of Bak1 alleviates microglial necroptosis and neuroinflammation after experimental subarachnoid hemorrhage
Journal: J Neurochem | Authors: Xiancheng Qiu | Species: mouse | Application: WB - THBS1 Induces Dysfunction of Ovarian Granulosa Cells in Patients with Polycystic Ovary Syndrome by Activating the TGF-β/Smad Pathway.
Journal: Biomedicines | Authors: Jiajing He | Species: rat,human | Application: IHC,CoIP - Beneficial effects of electro-acupuncture treatment in photothrombotic ischemia model rats by remodeling neurovascular unit.
Journal: Brain Res Bull | Authors: Zuanfang Li | Species: rat | Application: WB - THBS1 Induces Dysfunction of Ovarian Granulosa Cells in Patients with Polycystic Ovary Syndrome by Activating the TGF-β/Smad Pathway.
Journal: Biomedicines | Authors: Jiajing He | Species: rat,human | Application: IHC,CoIP - Beneficial effects of electro-acupuncture treatment in photothrombotic ischemia model rats by remodeling neurovascular unit.
Journal: Brain Res Bull | Authors: Zuanfang Li | Species: rat | Application: WB - hsa_circ_0007919 promotes pancreatic cancer metastasis by modulating Sp1-mediated THBS1 transcription
Journal: FASEB J | Authors: Xiao Ma | Species: mouse,human | Application: WB,IHC - N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance
Journal: Autoimmunity | Authors: Lin-Na Li | Species: human | Application: WB - MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis
Journal: Cancer Res Treat | Authors: Yang Yang | Species: human | Application: WB - Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy.
Journal: Front Neurosci | Authors: Zijun Lin | Species: human | Application: WB - Discovery and confirmation of crucial genes associated with radiation-induced heart disease
Journal: Int J Med Sci | Authors: Chuanbin Liu | Species: mouse | Application: IHC - Suppression of distal-less homeobox 3 increased alveolar bone mass in mouse tooth socket by targeting thrombospondin 1.
Journal: J Dent Sci | Authors: Chang Diao | Species: mouse | Application: IHC - THBS1 promotes angiogenesis and accelerates ESCC malignant progression by the HIF-1/VEGF signaling pathway
Journal: Cell Biol Int | Authors: Xiao Zhou | Species: mouse,human | Application: WB,IHC - Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats
Journal: Diabetes Metab Syndr Obes | Authors: Yu Ni | Species: rat | Application: IHC,IF - Inhibition of THBS1 axis contributes to the antitumor effect of PA-MSHA in anaplastic thyroid cancer
Journal: Exp Cell Res | Authors: Zhe Li | Species: mouse,human | Application: WB,IHC,IF - tRNA-Derived Fragment tRF-17-79MP9PP Attenuates Cell Invasion and Migration via THBS1/TGF-β1/Smad3 Axis in Breast Cancer.
Journal: Front Oncol | Authors: Dongping Mo | Species: human | Application: WB - Discovery of small extracellular vesicle proteins from human serum for liver cirrhosis and liver cancer.
Journal: Biochimie | Authors: Asad Uzzaman | Species: human | Application: WB - The protective effect of HOXA5 on carotid atherosclerosis occurs by modulating the vascular smooth muscle cell phenotype.
Journal: Mol Cell Endocrinol | Authors: Yuchen Jing | Species: mouse | Application: WB - Zedoarondiol Inhibits Neovascularization in Atherosclerotic Plaques of ApoE-/- Mice by Reducing Platelet Exosomes-Derived MiR-let-7a
Journal: Chin J Integr Med | Authors: Bei-Li Xie | Species: mouse,human | Application: WB,IHC - THBS1 Contributes to Chemoresistance in Ovarian Clear Cell Carcinoma via Promoting Epithelial-Mesenchymal Transition.
Journal: J Gene Med | Authors: Dongdong Ye | Species: human | Application: WB,IHC - Transcriptomic characterization revealed that METTL7A inhibits melanoma progression via the p53 signaling pathway and immunomodulatory pathway
Journal: PeerJ | Authors: Duoli Zhang | Species: human | Application: WB - Potential targets for synergistic bipolar irreversible electroporation in tumor suppression through transcriptomics and proteomics analysis
Journal: Gene | Authors: Yancheng Wang | Species: mouse | Application: WB - Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia.
Journal: Oral Surg Oral Med Oral Pathol Oral Radiol | Authors: Huilin Guo | Species: human | Application: WB - Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study.
Journal: Oral Surg Oral Med Oral Pathol Oral Radiol | Authors: Huilin Guo | Species: human | Application: WB - IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway.
Journal: J Oral Pathol Med | Authors: Jie Xu | Species: human | Application: WB - Growth hormone therapy promotes bone growth in idiopathic short stature children by activating the IGF-1 pathway via IGFBP2-mediated inhibition of THBS1.
Journal: In Vitro Cell Dev Biol Anim | Authors: Haiyan Liu | Species: human | Application: WB - Targeting Thbs1 reduces bladder remodeling caused by partial bladder outlet obstruction via the FGFR3/p-FGFR3 pathway
Journal: Neurourol Urodyn | Authors: Jun Long | Species: human | Application: WB - High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats
Journal: Acta Histochem Cytochem | Authors: Ryosuke Nakanishi | Species: Rat - Identification of shared genes and immune profiling for pathological scar
Journal: J Burn Care Res | Authors: Shuqian Dou | Species: human | Application: IHC - MiR-18a-5p acts as a novel serum biomarker for venous malformation and promotes angiogenesis by regulating the thrombospondin-1/P53 signaling axis.
Journal: Am J Transl Res | Authors: Liming Zhang | Species: mouse,human | Application: WB,IHC - miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1.
Journal: Comput Math Methods Med | Authors: Gang Xu | Species: human | Application: WB - The PTPN6-THBS1 axis drives immunosuppression in renal cell carcinoma by polarizing M2 tumor-associated macrophages.
Journal: Discov Oncol | Authors: Dingkun Hou | Species: mouse | Application: WB - Identification of Wnt/β-catenin- and immune-related genes in hepatic ischemia-reperfusion injury using bulk transcriptomics, single-cell RNA sequencing, and a murine model.
Journal: Front Immunol | Authors: Zhengjun Zhou - Cordycepin protects against simulated microgravity-induced osteogenic impairment involving ferroptosis-related signaling.
Journal: Biochem Pharmacol | Authors: Penghui Nie
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