STAU1 Polyclonal antibody 14225-1-AP proteintech
$149.00
In stock
SKU
14225-1-AP
| Catalog Number | 14225-1-AP |
| Synonyms | Double-stranded RNA-binding protein Staufen homolog 1, STAU |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, pig, monkey |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, RIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, human brain tissue, K-562 cells, mouse brain tissue, Jurkart cells |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HepG2 cells, human brain tissue, K-562 cells, mouse brain tissue, Jurkart cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5464 Product name: Recombinant human STAU1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 227-577 aa of BC050432 Sequence: GEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC Predict reactive species |
| Full Name | staufen, RNA binding protein, homolog 1 (Drosophila) |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC050432 |
| Gene Symbol | STAU1 |
| Gene ID (NCBI) | 6780 |
| RRID | AB_2302744 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95793 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for STAU1 antibody 14225-1-AP | Download protocol |
| IHC protocol for STAU1 antibody 14225-1-AP | Download protocol |
| IP protocol for STAU1 antibody 14225-1-AP | Download protocol |
| WB protocol for STAU1 antibody 14225-1-AP | Download protocol |
| human | WB Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Authors - Evan P Booy View Article |
| mouse,human | WB,RIP,IHC Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk Authors - Pingting Ying View Article |
| Citations | 28 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. There are two isoforms of STAU1 protein and the molecular weight of two isoforms are repectively 63 kDa and 55 kDa Protocols Product Specific Protocols IF protocol for STAU1 antibody 14225-1-AP Download protocol IHC protocol for STAU1 antibody 14225-1-AP Download protocol IP protocol for STAU1 antibody 14225-1-AP Download protocol WB protocol for STAU1 antibody 14225-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1.
Journal: Nature | Authors: Yoichiro Sugimoto KD Validated | Species: human | Application: WB - NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.
Journal: Cell Metab | Authors: Mi Kyung Park | Species: human | Application: WB - Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.
Journal: Mol Cell | Authors: Shih-Han Lee | Application: RIP - Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk
Journal: Nat Commun | Authors: Pingting Ying | Species: mouse,human | Application: WB,RIP,IHC - Axonal Transport of TDP-43 mRNA Granules Is Impaired by ALS-Causing Mutations.
Journal: Neuron | Authors: Nael H Alami | Species: human | Application: WB - Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.
Journal: Nucleic Acids Res | Authors: Evan P Booy | Species: human | Application: WB - Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function
Journal: Redox Biol | Authors: Fanglin Niu | Species: mouse,human | Application: WB - Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.
Journal: Mol Cell | Authors: Shih-Han Lee | Application: RIP - Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.
Journal: Nucleic Acids Res | Authors: Evan P Booy | Species: human | Application: WB - Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.
Journal: Nucleic Acids Res | Authors: Deepak Kumar Yadav | Species: human | Application: WB - PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.
Journal: Cell Death Dis | Authors: Rui Su | Species: human | Application: WB - LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation.
Journal: Cancer Lett | Authors: Chen Chen | Species: human | Application: RIP,IF - Annexin A7 enhances TIA1 axonal trafficking to counteract pathological aggregation in neurons.
Journal: EMBO J | Authors: Yu Feng | Species: rat | Application: IF - RNA-binding protein GIGYF2 orchestrates hepatic insulin resistance through STAU1/PTEN-mediated disruption of the PI3K/AKT signaling cascade
Journal: Mol Med | Authors: Ziwei Lv | Species: mouse,human | Application: WB - DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs
Journal: Cell Rep | Authors: Weili Miao | Species: human | Application: WB - Excessive STAU1 condensate drives mTOR translation and autophagy dysfunction in neurodegeneration
Journal: J Cell Biol | Authors: Ruiqian Zhao | Species: mouse,monkey,human | Application: WB,IF - Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.
Journal: Protein Sci | Authors: Andrea Tripepi | Species: human | Application: WB - STAU1-mediated CNBP mRNA degradation by LINC00665 alters stem cell characteristics in ovarian cancer
Journal: Biol Direct | Authors: Xiaofang Liu | Species: human | Application: WB - Vinca alkaloid drugs promote stress-induced translational repression and stress granule formation.
Journal: Oncotarget | Authors: Witold Szaflarski - CCAT1 attenuates intermittent hypoxia associated endothelial cell pyroptosis through STAU1-mediated IRF1 mRNA degradation.
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Wen Liu | Species: human | Application: WB - STAU1 exhibits oncogenic characteristics and modulates alternative splicing and gene expression in lung adenocarcinoma cells
Journal: Sci Rep | Authors: Ling Zhang | Species: human | Application: WB - Lipopolysaccharide enhances ADAR2 which drives Hirschsprung's disease by impairing miR-142-3p biogenesis.
Journal: J Cell Mol Med | Authors: Lei Peng | Species: human | Application: WB - STAU1 binds to IBDV genomic double-stranded RNA and promotes viral replication via attenuation of MDA5-dependent β interferon induction.
Journal: FASEB J | Authors: Chengjin Ye - RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.
Journal: Mol Ther Oncolytics | Authors: Shuyuan Shen | Species: human | Application: RIP - Identification of whole-cell dsRNA-binding proteins by phase separation
Journal: RNA Biol | Authors: Zhixiang Yang | Species: pig,mouse,human | Application: WB - The RNA-binding protein MSI2 controls blood-tumor barrier permeability via LINC00667-Mediated IRF6 mRNA decay.
Journal: J Biol Chem | Authors: Rui Gao | Species: human | Application: WB - Methods for the characterization of stress granules in virus infected cells.
Journal: Methods | Authors: Marc D Panas - Long noncoding RNA TINCR is a novel regulator of human bronchial epithelial cell differentiation state.
Journal: Physiol Rep | Authors: Norihito Omote | Species: human | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
