STUB1 Polyclonal antibody 29751-1-AP proteintech
$149.00
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29751-1-AP
| Catalog Number | 29751-1-AP |
| Synonyms | CHIP, Antigen NY-CO-7, Carboxy terminus of Hsp70-interacting protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, PC-12 cells, mouse kidney tissue, rat kidney tissue |
| Tested Applications — Positive IHC detected in | rat lung tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, PC-12 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | rat lung tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag31335 Product name: Recombinant human STUB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 73-303 aa of BC007545 Sequence: MQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY Predict reactive species |
| Full Name | STIP1 homology and U-box containing protein 1 |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC007545 |
| Gene Symbol | STUB1 |
| Gene ID (NCBI) | 10273 |
| RRID | AB_2923603 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for STUB1 antibody 29751-1-AP | Download protocol |
| WB protocol for STUB1 antibody 29751-1-AP | Download protocol |
| rat | WB Eur J Pharmacol Sodium Houttuyfonate Alleviates Monocrotaline-induced Pulmonary Hypertension by Regulating Canonical Transient Receptor Potential Channel Proteins via CHIP. Authors - Suya Zhang KD Validated View Article |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) Protocols Product Specific Protocols IHC protocol for STUB1 antibody 29751-1-AP Download protocol WB protocol for STUB1 antibody 29751-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Sodium Houttuyfonate Alleviates Monocrotaline-induced Pulmonary Hypertension by Regulating Canonical Transient Receptor Potential Channel Proteins via CHIP.
Journal: Eur J Pharmacol | Authors: Suya Zhang KD Validated | Species: rat | Application: WB
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