PLAP Polyclonal antibody 18507-1-AP proteintech
$149.00
In stock
SKU
18507-1-AP
| Catalog Number | 18507-1-AP |
| Synonyms | ALP, ALPP, EC:3.1.3.1, PALP, PLAP-1 |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human placenta tissue, mouse liver tissue, HepG2 cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | human placenta tissue, mouse liver tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag13361 Product name: Recombinant human ALPP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 199-535 aa of BC009647 Sequence: SARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPGRSVVPALLPLLAGTLLLLETATAP Predict reactive species |
| Full Name | alkaline phosphatase, placental (Regan isozyme) |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC009647 |
| Gene Symbol | PLAP |
| Gene ID (NCBI) | 250 |
| RRID | AB_10644334 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05187 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PLAP antibody 18507-1-AP | Download protocol |
| IP protocol for PLAP antibody 18507-1-AP | Download protocol |
| WB protocol for PLAP antibody 18507-1-AP | Download protocol |
| mouse | WB Mol Cell Endocrinol Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis. Authors - Jinhui Wu View Article |
| rat | WB,IHC Int J Biol Macromol Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides Authors - Taojing Yu View Article |
| human | IHC ACS Biomater Sci Eng 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model. Authors - Song Shen View Article |
| Citations | 20 |
| Dilutions | WB : 1:1000-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies. Protocols Product Specific Protocols IHC protocol for PLAP antibody 18507-1-AP Download protocol IP protocol for PLAP antibody 18507-1-AP Download protocol WB protocol for PLAP antibody 18507-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway
Journal: J Nanobiotechnology | Authors: Yue Li | Species: mouse | Application: WB - Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides
Journal: Int J Biol Macromol | Authors: Taojing Yu | Species: rat | Application: WB,IHC - Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.
Journal: Aging (Albany NY) | Authors: Jie Xu | Species: mouse | Application: WB - 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model.
Journal: ACS Biomater Sci Eng | Authors: Song Shen | Species: human | Application: IHC - Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis.
Journal: Mol Cell Endocrinol | Authors: Jinhui Wu | Species: mouse | Application: WB - PMID: 32119967
Journal: J Dent | Authors: Yunxia Zhang | Application: WB - Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides
Journal: Int J Biol Macromol | Authors: Taojing Yu | Species: rat | Application: WB,IHC - Biomimetic cryogel promotes the repair of osteoporotic bone defects through altering the ROS niche via down-regulating the ROMO1
Journal: Int J Biol Macromol | Authors: Hai Liu | Species: rat | Application: WB - Mechanically induced M2 macrophages are involved in bone remodeling of the midpalatal suture during palatal expansion
Journal: Prog Orthod | Authors: Lan Li | Species: rat,mouse | Application: WB - 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model.
Journal: ACS Biomater Sci Eng | Authors: Song Shen | Species: human | Application: IHC - Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.
Journal: Aging (Albany NY) | Authors: Jie Xu | Species: mouse | Application: WB - Liraglutide regulates bone destruction and exhibits anti-inflammatory effects in periodontitis in vitro and in vivo.
Journal: J Dent | Authors: Yunxia Zhang | Application: WB - Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment
Journal: Front Endocrinol (Lausanne) | Authors: Yunpeng Wang | Species: human | Application: IHC - Long non-coding RNA NRIR inhibits osteogenesis in peri-implantitis by promoting NLRP3 inflammasome-mediated macrophage pyroptosis
Journal: Int Immunopharmacol | Authors: Lan Wang | Species: mouse | Application: WB - Fracture-Induced Immunological Cascades Trigger Rapid Systemic Bone Loss via Osteocyte-Regulated Osteoclastogenesis
Journal: Immunotargets Ther | Authors: Lipeng Sun - Role and mechanism of Actein on condylar bone metabolism in APOE deletion-induced osteoporotic mice
Journal: Bone | Authors: Linyi Zhou | Application: IHC - METTL3-mediated m6A modification of circSTAT6 modulates miR-188-3p/Beclin1 axis to promote osteogenic differentiation of mesenchymal stem cells
Journal: J Orthop Surg Res | Authors: Yue Luo | Species: human | Application: WB - Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis.
Journal: Mol Cell Endocrinol | Authors: Jinhui Wu | Species: mouse | Application: WB - New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor.
Journal: Amino Acids | Authors: Halina Jurkowska | Species: human | Application: WB - Influence of METTL3 knockdown on PDLSC osteogenesis in E. coli LPS-induced inflammation
Journal: Oral Dis | Authors: Hang Chen | Species: human | Application: WB
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