SURF4 Polyclonal antibody 11599-1-AP proteintech
$149.00
In stock
SKU
11599-1-AP
| Catalog Number | 11599-1-AP |
| Synonyms | surfeit 4, SURF-4, SURF 4, ERV29 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse liver tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2175 Product name: Recombinant human SURF4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC018741 Sequence: MGQNDLMGTAEDFADQFLRVTKQYLPHVARLCLISTFLEDGIRMWFQWSEQRDYIDTTWNCGYLLASSFVFLNLLGQLTGCVLVLSRNFVQYACFGLFGIIALQTIAYSILWDLKFLMRNLALGGGLLLLLAESRSEGKSMFAGVPTMRESSPKQYMQLGGRVLLVLMFMTLLHFDASFFSIVQNIVGTALMILVAIGFKTKLAALTLVVWLFAINVYFNAFWTIPVYKPMHDFLKYDFFQTMSVIGGLLLVVALGPGGVSMDEKKKEW Predict reactive species |
| Full Name | surfeit 4 |
| Calculated Molecular Weight | 269 aa, 30 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC018741 |
| Gene Symbol | SURF4 |
| Gene ID (NCBI) | 6836 |
| RRID | AB_2877782 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15260 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for SURF4 antibody 11599-1-AP | Download protocol |
| WB protocol for SURF4 antibody 11599-1-AP | Download protocol |
| human | IHC Cancer Immunol Immunother Identification of SURF4 and RALGAPA1 as promising therapeutic targets in glioblastoma and pan-cancer through integrative multi-omics, CRISPR-Cas9 screening and prognostic meta-analysis Authors - Fei Wang View Article |
| mouse | WB Biochim Biophys Acta Mol Cell Biol Lipids Inducible global knockout of surfeit locus protein 4 in adult mice results in hypolipidemia, intestinal lipid accumulation, liver injury, and increased mortality Authors - Wei Chen KO Validated View Article |
| Fiona (Verified Customer) (10-19-2021) | Used for immunofluorescence on HeLa cells. Good, clear signal with minimal background at 1:500 without antigen retrieval. Applications: Immunofluorescence Primary Antibody Dilution: 1:500 |
| Citations | 4 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment
Journal: J Transl Med | Authors: Guohong Liu | Species: human | Application: IHC - Inducible global knockout of surfeit locus protein 4 in adult mice results in hypolipidemia, intestinal lipid accumulation, liver injury, and increased mortality
Journal: Biochim Biophys Acta Mol Cell Biol Lipids | Authors: Wei Chen KO Validated | Species: mouse | Application: WB - [High expression of SURF4 promotes migration, invasion and proliferation of gastric cancer cells by inhibiting tight junction proteins].
Journal: Nan Fang Yi Ke Da Xue Xue Bao | Authors: WANG Ziliang | Species: human | Application: WB,IHC - Identification of SURF4 and RALGAPA1 as promising therapeutic targets in glioblastoma and pan-cancer through integrative multi-omics, CRISPR-Cas9 screening and prognostic meta-analysis
Journal: Cancer Immunol Immunother | Authors: Fei Wang | Species: human | Application: IHC
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