SUZ12 Polyclonal antibody 20366-1-AP proteintech
$149.00
In stock
SKU
20366-1-AP
| Catalog Number | 20366-1-AP |
| Synonyms | ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160 |
| Host | Rabbit |
| Reactivity | human, rat and More (1) |
| Reactivity | human, rat, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, CoIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | suppressor of zeste 12 homolog (Drosophila) |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | NM_015355 |
| Gene Symbol | SUZ12 |
| Gene ID (NCBI) | 23512 |
| RRID | AB_10694152 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15022 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| IP protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| WB protocol for SUZ12 antibody 20366-1-AP | Download protocol |
| human | WB Am J Cancer Res N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex. Authors - Jing Sun View Article |
| Nikhil (Verified Customer) (01-07-2022) | HCT116 whole cell extract, 20micro/lane. Lane 2-4 transient expression of EZH2 Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HCT116 SUZ12 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:20366-1-AP) |
| Citations | 21 |
| Dilutions | WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Protocols Product Specific Protocols IHC protocol for SUZ12 antibody 20366-1-AP Download protocol IP protocol for SUZ12 antibody 20366-1-AP Download protocol WB protocol for SUZ12 antibody 20366-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer
Journal: Mol Cancer | Authors: Rashmi Bharti | Species: human | Application: WB - TRACE generates fluorescent human reporter cell lines to characterize epigenetic pathways.
Journal: Mol Cell | Authors: Iva A Tchasovnikarova KD Validated | Species: human | Application: WB - A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.
Journal: EMBO J | Authors: Xu Wang | Species: human | Application: WB - Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.
Journal: Eur J Med Chem | Authors: Cheng Wang | Species: human | Application: WB - Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.
Journal: Sci Rep | Authors: Masamitsu N Asaka | Species: human | Application: WB - N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.
Journal: Am J Cancer Res | Authors: Jing Sun | Species: human | Application: WB - PRMT1-mediated EZH2 methylation promotes breast cancer cell proliferation and tumorigenesis.
Journal: Cell Death Dis | Authors: Zhongwei Li | Species: human | Application: WB,IP - SUMO E3 ligase MUL1 inhibits lymph node metastasis of bladder cancer by mediating mitochondrial HSPA9 translocation
Journal: Int J Biol Sci | Authors: Jilin Wu | Species: mouse,human | Application: WB,IP,IHC - AZD9291 inactivates the PRC2 complex to mediate tumor growth inhibition.
Journal: Acta Pharmacol Sin | Authors: Kai-Li Zhang | Species: human | Application: WB - A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.
Journal: EMBO J | Authors: Xu Wang | Species: human | Application: WB - Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.
Journal: Eur J Med Chem | Authors: Cheng Wang | Species: human | Application: WB - CircARAP2 controls sMICA-induced NK cell desensitization by erasing CTCF/PRC2-induced suppression in early endosome marker RAB5A
Journal: Cell Mol Life Sci | Authors: Feifei Guo | Species: human | Application: CoIP - MAPK15 Attenuates Intestinal Epithelial Cell Senescence-Like Phenotype to Regulate Gut Homeostasis and Inflammation by Phosphorylating Suz12 to Suppress Drp1-Mediated Mitochondrial Fission Damage.
Journal: Inflammation | Authors: Juan Li | Species: human, mouse | Application: WB,CoIP - Cyclohexene oxide CA, a derivative of zeylenone, exhibits anti-cancer activity in glioblastoma by inducing G0/G1 phase arrest through interference with EZH2
Journal: Front Pharmacol | Authors: Rui Su | Species: human | Application: WB - Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction
Journal: mSystems | Authors: Wei Hu | Species: human | Application: WB - TRPS1 Suppresses Breast Cancer Epithelial-mesenchymal Transition Program as a Negative Regulator of SUZ12.
Journal: Transl Oncol | Authors: Jing Hu | Species: human | Application: IHC - Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.
Journal: Sci Rep | Authors: Masamitsu N Asaka | Species: human | Application: WB - Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.
Journal: Cell Signal | Authors: Zijie Zhang | Species: human | Application: WB - Cadmium-induced annulus fibrosus cell senescence contributes to intervertebral disc degeneration via the JNK/p53 signaling pathway
Journal: Iran J Basic Med Sci | Authors: Xin Liu | Species: Rat - N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex.
Journal: Am J Cancer Res | Authors: Jing Sun | Species: human | Application: WB - The m6 A reader YTHDC2 regulates UVB-induced DNA damage repair and histone modification
Journal: Photochem Photobiol | Authors: Zizhao Yang | Species: human | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
