ChromoTek Spot-Label® Alexa Fluor® 488 ebAF488 proteintech
$179.00
In stock
SKU
ebAF488
| Synonyms | PDRVRAVSHWSS, Spot, Spot tag, Spot-Label, SPOT-tag |
| Host | Alpaca |
| Formulation | United States |
| Applications | IF |
| Host / Isotype | IF |
| Specificity/Target | Spot-Tag ® (PDRVRAVSHWSS) |
| Conjugate | Alexa Fluor® 488 |
| Physical State | Liquid |
| Suggested Dilution | IF: 1:800 |
| Type | Nanobody |
| Class | Recombinant |
| Clonality | Monoclonal |
| Clone | CTK0216 |
| Calculated MW | 30.3 kDa |
| RRID | AB_2889374 |
| Immunogen | Beta-catenin, engineered epitope |
| Format | Recombinant alpaca single domain antibody, bivalent |
| Affinity (K D ) | Dissociation constant K D of 6 nM |
| DOL | 1 fluorophore per bivalent Nanobody, site-directed conjugation |
| Spot-Tag® Origin | Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin |
| Cross Reactivity | The Spot-Nanobody shows minimal cross reactivity with beta-catenin. |
| Excitation/ Emission | Excitation max: 490 nm, Emission max: 525 nm |
| Storage Buffer | PBS, 5 mM EDTA, preservative: 0.09 % sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light. |
| Size | 10 μL; 50 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
| IF | PLoS Genet C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1. Authors - Keshav Jha View Article |
Description
Immunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy. • Capture and detection tag without compromises in applications • Higher image resolution • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments • Strong avidity effect from bivalent form of Spot-VHH • Fulfills highest requirements on antibody validation: structure and function characterized
Publications
- The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans
Journal: EMBO Rep | Authors: Prabhu Sankaralingam | Species: IF | Application: EMBO Rep The kinase ZYG-1 phosphorylates the cartwheel protein SAS-5 to drive centriole assembly in C. elegans Authors - Prabhu Sankaralingam View Article - C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1.
Journal: PLoS Genet | Authors: Keshav Jha | Species: IF | Application: PLoS Genet C. elegans SAS-1 ensures centriole integrity and ciliary function, and operates with SSNA-1. Authors - Keshav Jha View Article
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