TAF11 Polyclonal antibody 16114-1-AP proteintech
$149.00
In stock
SKU
16114-1-AP
| Catalog Number | 16114-1-AP |
| Synonyms | TFIID subunit p30 beta, TAFII-28, TAFII28, TAFII 28, TAF2I |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, HeLa cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:200-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9039 Product name: Recombinant human TAF11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC021972 Sequence: MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMPPLQPKHMREAVRRLKSKGQIPNSKHKKIIFF Predict reactive species |
| Full Name | TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28kDa |
| Calculated Molecular Weight | 211 aa, 23 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC021972 |
| Gene Symbol | TAF11 |
| Gene ID (NCBI) | 6882 |
| RRID | AB_2878220 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15544 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TAF11 antibody 16114-1-AP | Download protocol |
| IHC protocol for TAF11 antibody 16114-1-AP | Download protocol |
| IP protocol for TAF11 antibody 16114-1-AP | Download protocol |
| WB protocol for TAF11 antibody 16114-1-AP | Download protocol |
| mouse | WB Int Immunopharmacol DQ690018 regulated HIF1α expression and subsequently mediates macrophage polarization by inhibiting Taf11 mRNA 2'-O-methylation modification. Authors - Kai Liu View Article |
| Citations | 2 |
| Dilutions | WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TAF11, also named as Transcription initiation factor TFIID 28 kDa subunit, is a 211 amino acid protein, which Belongs to the TAF11 family. TAF11 as a core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 . The calcualted molecular weight of TAF11 is 23 kDa, but modified TAF11 is about 28kDa. ( PMID: 10336414) Protocols Product Specific Protocols IF protocol for TAF11 antibody 16114-1-AP Download protocol IHC protocol for TAF11 antibody 16114-1-AP Download protocol IP protocol for TAF11 antibody 16114-1-AP Download protocol WB protocol for TAF11 antibody 16114-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The transcription factor IID subunit Taf13 is dispensable for TATA binding protein promoter recruitment and RNA polymerase II transcription
Journal: iScience | Authors: Igor Martianov | Species: mouse | Application: WB - DQ690018 regulated HIF1α expression and subsequently mediates macrophage polarization by inhibiting Taf11 mRNA 2'-O-methylation modification.
Journal: Int Immunopharmacol | Authors: Kai Liu | Species: mouse | Application: WB
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