TAF1B Polyclonal antibody 12818-1-AP proteintech
$149.00
In stock
SKU
12818-1-AP
| Catalog Number | 12818-1-AP |
| Synonyms | RAF1B, RAFI63, RNA polymerase I-specific TBP-associated factor 63 kDa, SL1, TAFI63 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, NIH/3T3 cells |
| Tested Applications — Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | HeLa cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3267 Product name: Recombinant human TAF1B protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-346 aa of BC018137 Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIA Predict reactive species |
| Full Name | TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa |
| Calculated Molecular Weight | 588 aa, 69 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC018137 |
| Gene Symbol | TAF1B |
| Gene ID (NCBI) | 9014 |
| RRID | AB_10644160 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53T94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TAF1B antibody 12818-1-AP | Download protocol |
| WB protocol for TAF1B antibody 12818-1-AP | Download protocol |
| human | WB Oncol Lett Identification of a novel FOXO3‑associated prognostic model in hepatocellular carcinoma Authors - Songmei Guan View Article |
| Citations | 2 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TAF1B is a component of the RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. It binds to rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex, and possibly directly through its interaction with RRN3 (PMID: 11250903; 11283244; 15970593; 21921199). Protocols Product Specific Protocols IHC protocol for TAF1B antibody 12818-1-AP Download protocol WB protocol for TAF1B antibody 12818-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.
Journal: Nat Commun | Authors: Yanqin Yu - Identification of a novel FOXO3‑associated prognostic model in hepatocellular carcinoma
Journal: Oncol Lett | Authors: Songmei Guan | Species: human | Application: WB
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