Multi-rAb® transgelin/SM22 Multi-Recombinant antibody RMX00045 proteintech
$449.00
In stock
SKU
RMX00045
| Catalog Number | RMX00045 |
| Synonyms | SM22, transgelin, 22 kDa actin-binding protein, Protein WS3-10, SM22-a |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue |
| Tested Applications — Positive IHC detected in | mouse heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse heart tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:20000-1:100000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue |
| Positive IHC detected in | mouse heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | transgelin |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 19-22 kDa |
| GenBank Accession Number | BC004927 |
| Gene Symbol | transgelin/SM22 |
| Gene ID (NCBI) | 6876 |
| RRID | AB_3750097 |
| Conjugate | Unconjugated |
| Purification Method | N/A |
| UNIPROT ID | Q01995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for transgelin/SM22 antibody RMX00045 | Download protocol |
| IHC protocol for transgelin/SM22 antibody RMX00045 | Download protocol |
| WB protocol for transgelin/SM22 antibody RMX00045 | Download protocol |
| Citations | - |
| Dilutions | WB : 1:20000-1:100000 IHC : 1:2000-1:8000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Multi-Recombinant |
| Clone Number | - |
| Conjugation | Unconjugated |
The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can cross-react with other two transgenes based on the sequence similarity. Protocols Product Specific Protocols IF protocol for transgelin/SM22 antibody RMX00045 Download protocol IHC protocol for transgelin/SM22 antibody RMX00045 Download protocol WB protocol for transgelin/SM22 antibody RMX00045 Download protocol Standard Protocols Click here to view our Standard Protocols
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