TBC1D1 Polyclonal antibody 22124-1-AP proteintech
$149.00
In stock
SKU
22124-1-AP
| Catalog Number | 22124-1-AP |
| Synonyms | KIAA1108, TBC, TBC1, TBC1 domain family member 1, TBC1D1 |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, bovine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | Jurkat cells |
| Tested Applications — Positive IHC detected in | mouse kidney tissue, human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse kidney tissue, human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, bovine |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5406 Product name: Recombinant human TBC1D1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 813-1168 aa of BC050321 Sequence: LAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD Predict reactive species |
| Full Name | TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 |
| Calculated Molecular Weight | 133 kDa |
| Observed Molecular Weight | 150-165 kDa |
| GenBank Accession Number | BC050321 |
| Gene Symbol | TBC1D1 |
| Gene ID (NCBI) | 23216 |
| RRID | AB_2827874 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86TI0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TBC1D1 antibody 22124-1-AP | Download protocol |
| IP protocol for TBC1D1 antibody 22124-1-AP | Download protocol |
| WB protocol for TBC1D1 antibody 22124-1-AP | Download protocol |
| human | WB J Physiol The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. Authors - Magnus R Larsen View Article |
| mouse | WB J Biol Chem Secreted EMC10 inhibits muscle GLUT4 activity and glucose uptake in mice Authors - Shuoshuo Jin View Article |
| Q (Verified Customer) (06-01-2021) | Excellent to detect TBC1D1 in postmortem human samples with WB with the right MW. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: postmortem human brain lysate TBC1D1 Antibody Western Blot validation (1:2000 dilution) in postmortem human brain lysate (Cat no:22124-1-AP) |
| Citations | 13 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise
Journal: Nat Metab | Authors: Jonas M Kristensen | Species: human | Application: WB - Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle.
Journal: Diabetes | Authors: Jeppe K Larsen | Species: human | Application: WB,IP - Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation.
Journal: Mol Nutr Food Res | Authors: Kang Liu | Species: mouse | Application: WB - The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres.
Journal: J Physiol | Authors: Magnus R Larsen | Species: human | Application: WB - Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities.
Journal: J Biol Chem | Authors: Hiroyasu Hatakeyama - Secreted EMC10 inhibits muscle GLUT4 activity and glucose uptake in mice
Journal: J Biol Chem | Authors: Shuoshuo Jin | Species: mouse | Application: WB - Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation.
Journal: Mol Nutr Food Res | Authors: Kang Liu | Species: mouse | Application: WB - Secreted EMC10 inhibits muscle GLUT4 activity and glucose uptake in mice
Journal: J Biol Chem | Authors: Shuoshuo Jin | Species: mouse | Application: WB - Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities.
Journal: J Biol Chem | Authors: Hiroyasu Hatakeyama - Stevioside Ameliorates Palmitic Acid-Induced Abnormal Glucose Uptake via the PDK4/AMPK/TBC1D1 Pathway in C2C12 Myotubes
Journal: Endocrinol Diabetes Metab | Authors: Changfa Zhang | Species: mouse | Application: WB - Coumarins ameliorate diabetogenic action of dexamethasone via Akt activation and AMPK signaling in skeletal muscle.
Journal: J Pharmacol Sci | Authors: Zejun Mo | Species: mouse | Application: WB - GPR30 selective agonist G-1 induced insulin resistance in ovariectomized mice on high fat diet and its mechanism
Journal: Biochem Biophys Res Commun | Authors: Congcong Lu | Species: mouse | Application: WB - TBC1D1 represses glioma progression by altering the integrity of the cytoskeleton
Journal: Aging (Albany NY) | Authors: Jiahong Cai | Species: human | Application: WB,IHC
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