CoraLite®594-conjugated TBP Monoclonal antibody CL594-66166 proteintech
$189.00
In stock
SKU
CL594-66166
| Catalog Number | CL594-66166 |
| Synonyms | TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive IF/ICC detected in | A431 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IF/ICC detected in | A431 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12383 Product name: Recombinant human TBP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-338 aa of BC110341 Sequence: MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT Predict reactive species |
| Full Name | TATA box binding protein |
| Calculated Molecular Weight | 338 aa, 38 kDa |
| Observed Molecular Weight | mouse/rat 33-36 kDa and human 37-43kDa |
| GenBank Accession Number | BC110341 |
| Gene Symbol | TBP |
| Gene ID (NCBI) | 6908 |
| RRID | AB_2919956 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P20226 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL594 TBP antibody CL594-66166 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 2H3B2 |
| Conjugation | CoraLite®594 |
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa). Protocols Product Specific Protocols IF protocol for CL594 TBP antibody CL594-66166 Download protocol Standard Protocols Click here to view our Standard Protocols
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