TBRG4 Polyclonal antibody 16245-1-AP proteintech
$149.00
In stock
SKU
16245-1-AP
| Catalog Number | 16245-1-AP |
| Synonyms | CPR2, FAST kinase domain-containing protein 4, FASTKD4, KIAA0948, Protein TBRG4 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells, Hela cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : N/A |
| Positive WB detected in | HepG2 cells, HeLa cells, human liver tissue, mouse ovary tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, Hela cells |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : N/A |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9176 Product name: Recombinant human TBRG4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 280-631 aa of BC017235 Sequence: SYHLVQKPFSLTKDVLLDVAYAYGKLSFHQTQVSQRLATDLLSLMPSLTSGEVAHCAKSFALLKWLSLPLFEAFAQHVLNRAQDITLPHLCSVLLAFARLNFHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFLGGKSQKDQNTFQKLLHINATALLEYPEYSGPLLPASAVAPGPSALDRKVTPLQKELQETLKGLLGSADKGSLEVATQYGWVLDAEVLLDSDGEFLPVRDFVAPHLAQPTGSQSPPPGSKRLAFLRWEFPNFNSRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDKMRKAVAEELAK Predict reactive species |
| Full Name | transforming growth factor beta regulator 4 |
| Calculated Molecular Weight | 631 aa, 71 kDa |
| Observed Molecular Weight | 60 kDa, 71 kDa |
| GenBank Accession Number | BC017235 |
| Gene Symbol | TBRG4 |
| Gene ID (NCBI) | 9238 |
| RRID | AB_2271487 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969Z0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| IHC protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| IP protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| WB protocol for TBRG4 antibody 16245-1-AP | Download protocol |
| human | WB,IP,IF Spine J TBRG4 regulates ubiquitination of Beclin1 and participates in autophagy pathway to inhibit intervertebral disc degeneration Authors - Xilong Cui View Article |
| Erik (Verified Customer) (02-24-2021) | The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein Applications: Western Blot Cell Tissue Type: PANC1 and MIAPACA2 cell lines TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP) |
| Citations | 5 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:100-1:400 IF/ICC : N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing. Protocols Product Specific Protocols IF protocol for TBRG4 antibody 16245-1-AP Download protocol IHC protocol for TBRG4 antibody 16245-1-AP Download protocol IP protocol for TBRG4 antibody 16245-1-AP Download protocol WB protocol for TBRG4 antibody 16245-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The FASTK family proteins fine-tune mitochondrial RNA processing
Journal: PLoS Genet | Authors: Akira Ohkubo KO Validated | Species: human | Application: WB - High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma
Journal: BMC Cancer | Authors: Shanchun Tao | Species: human | Application: WB,IHC,IF,CoIP - Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.
Journal: Life Sci Alliance | Authors: Claudio Asencio | Species: human | Application: WB - The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA
Journal: Nucleic Acids Res | Authors: Xuan Yang KO Validated | Species: human | Application: WB,IP - TBRG4 regulates ubiquitination of Beclin1 and participates in autophagy pathway to inhibit intervertebral disc degeneration
Journal: Spine J | Authors: Xilong Cui | Species: human | Application: WB,IP,IF
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