TCF12/HEB Polyclonal antibody 14419-1-AP proteintech
$149.00
In stock
SKU
14419-1-AP
| Catalog Number | 14419-1-AP |
| Synonyms | TCF12, bHLHb20, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E box binding protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ChIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, HeLa cells, Raji cells |
| Tested Applications — Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HepG2 cells, HeLa cells, Raji cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5765 Product name: Recombinant human TCF12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 358-706 aa of BC050556 Sequence: SNPSTPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLKNRVEQQLHEHLQDAMSFLKDVCEQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSLVASSRSASMVGTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM Predict reactive species |
| Full Name | transcription factor 12 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC050556 |
| Gene Symbol | TCF12 |
| Gene ID (NCBI) | 6938 |
| RRID | AB_2198838 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99081 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TCF12/HEB antibody 14419-1-AP | Download protocol |
| WB protocol for TCF12/HEB antibody 14419-1-AP | Download protocol |
| mouse | WB,IHC,IF Stem Cells Tcf12, a Member of Basic Helix-loop-helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation in vitro and in vivo. Authors - Siqi Yi KD Validated View Article |
| human | WB iScience Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB. Authors - Holly Holliday View Article |
| Citations | 17 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424]. Protocols Product Specific Protocols IHC protocol for TCF12/HEB antibody 14419-1-AP Download protocol WB protocol for TCF12/HEB antibody 14419-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators
Journal: Nat Cell Biol | Authors: Bofeng Liu | Species: mouse | Application: IF - TCF12 is mutated in anaplastic oligodendroglioma.
Journal: Nat Commun | Authors: Karim Labreche | Species: human | Application: IHC - A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis.
Journal: Development | Authors: Sisu Han | Species: mouse | Application: IF - TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4
Journal: J Orthop Translat | Authors: Hui Zheng | Species: mouse | Application: WB,IF - Tcf12, a Member of Basic Helix-loop-helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation in vitro and in vivo.
Journal: Stem Cells | Authors: Siqi Yi KD Validated | Species: mouse | Application: WB,IHC,IF - Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.
Journal: iScience | Authors: Holly Holliday | Species: human | Application: WB - A novel TCF12-MAVS signaling axis in astrocytes promotes vincristine-induced neuropathic pain in male mice.
Journal: Brain Behav Immun | Authors: Lin Zhou - RNA-binding protein DHX9 promotes glioma growth and tumor-associated macrophages infiltration via TCF12
Journal: CNS Neurosci Ther | Authors: Liang Liu | Species: human | Application: WB - Tcf12, a Member of Basic Helix-loop-helix Transcription Factors, Mediates Bone Marrow Mesenchymal Stem Cell Osteogenic Differentiation in vitro and in vivo.
Journal: Stem Cells | Authors: Siqi Yi | Species: mouse | Application: WB,IHC,IF - TCF12 Activates TGFB2 Expression to Promote the Malignant Progression of Melanoma
Journal: Cancers (Basel) | Authors: Youjia Tian | Species: human | Application: IHC - Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.
Journal: iScience | Authors: Holly Holliday | Species: human | Application: WB - MSTN and TCF12 as Candidate Immunometabolic Signatures in Glioma-Associated Foam Cells: Insights from Integrated Multi-Omics Analysis.
Journal: Curr Issues Mol Biol | Authors: Xu Liu | Species: human | Application: IF - A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis.
Journal: Development | Authors: Sisu Han | Species: mouse | Application: IF - Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis.
Journal: BMC Genomics | Authors: Thorsen Kasper K | Species: human | Application: IHC - Triclosan inhibits human trophoblast cell migration via TCF12-mediated RASSF8 regulation.
Journal: Food Chem Toxicol | Authors: Guangzhu Dong | Species: human | Application: WB - Extensive gene-specific translational reprogramming in a model of B cell differentiation and Abl-dependent transformation.
Journal: PLoS One | Authors: Bates Jamie G JG | Species: human | Application: WB - Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalon
Journal: Neural Dev | Authors: Mary Jo Talley | Species: mouse | Application: IHC,IF
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