TCN2 Polyclonal antibody 12157-1-AP proteintech
$149.00
In stock
SKU
12157-1-AP
| Catalog Number | 12157-1-AP |
| Synonyms | D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, canine, cat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | BxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells |
| Tested Applications — Positive IHC detected in | mouse small intestine tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:300-1:600 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | BxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells |
| Positive IHC detected in | mouse small intestine tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, cat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2825 Product name: Recombinant human TCN2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC011239 Sequence: MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW Predict reactive species |
| Full Name | transcobalamin II; macrocytic anemia |
| Calculated Molecular Weight | 400 aa, 44 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC011239 |
| Gene Symbol | TCN2 |
| Gene ID (NCBI) | 6948 |
| RRID | AB_2303252 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TCN2 antibody 12157-1-AP | Download protocol |
| WB protocol for TCN2 antibody 12157-1-AP | Download protocol |
| canine, cat | IHC Oncotarget Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues. Authors - Annette M Sysel View Article |
| mouse | IF Int J Biol Macromol CD320 expression and apical membrane targeting in renal and intestinal epithelial cells. Authors - Yue Chen View Article |
| human | IHC Anticancer Res Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts. Authors - Sysel Annette M AM View Article |
| rat | WB Mol Pharm Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin Authors - Yuri Kinoshita View Article |
| Joseph (Verified Customer) (09-17-2020) | We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies Applications: Immunohistochemistry Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline Cell Tissue Type: human, canine and feline malignant various |
| Citations | 5 |
| Dilutions | WB : 1:300-1:600 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245) Protocols Product Specific Protocols IHC protocol for TCN2 antibody 12157-1-AP Download protocol WB protocol for TCN2 antibody 12157-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.
Journal: Oncotarget | Authors: Annette M Sysel | Species: canine, cat | Application: IHC - CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.
Journal: Int J Biol Macromol | Authors: Yue Chen | Species: mouse | Application: IF - Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.
Journal: Anticancer Res | Authors: Sysel Annette M AM | Species: human | Application: IHC - Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin
Journal: Mol Pharm | Authors: Yuri Kinoshita | Species: rat | Application: WB - TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation.
Journal: FASEB J | Authors: Jing-Kai Xu
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