TCP1 alpha Polyclonal antibody 10320-1-AP proteintech
$149.00
In stock
SKU
10320-1-AP
| Catalog Number | 10320-1-AP |
| Synonyms | TCP1, CCT alpha, CCT1, CCTa, CCT-alpha |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, mouse heart tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, NCI-H1299 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, human colon tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, mouse heart tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, NCI-H1299 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0389 Product name: Recombinant human TCP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 277-553 aa of BC000665 Sequence: KILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGA Predict reactive species |
| Full Name | t-complex 1 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC000665 |
| Gene Symbol | TCP1 |
| Gene ID (NCBI) | 6950 |
| RRID | AB_10694136 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17987 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for TCP1 alpha antibody 10320-1-AP | Download protocol |
| IF protocol for TCP1 alpha antibody 10320-1-AP | Download protocol |
| IHC protocol for TCP1 alpha antibody 10320-1-AP | Download protocol |
| IP protocol for TCP1 alpha antibody 10320-1-AP | Download protocol |
| WB protocol for TCP1 alpha antibody 10320-1-AP | Download protocol |
| human | WB J Cell Biol CEP55 promotes cilia disassembly through stabilizing Aurora A kinase. Authors - Yu-Cheng Zhang View Article |
| mouse,human | WB Front Cell Dev Biol TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis Authors - Wen-Shuai Tang View Article |
| mouse | IP Virology Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex. Authors - Sandhya Gopal View Article |
| Citations | 13 |
| Dilutions | WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:800-1:3200 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
0 Protocols Product Specific Protocols FC protocol for TCP1 alpha antibody 10320-1-AP Download protocol IF protocol for TCP1 alpha antibody 10320-1-AP Download protocol IHC protocol for TCP1 alpha antibody 10320-1-AP Download protocol IP protocol for TCP1 alpha antibody 10320-1-AP Download protocol WB protocol for TCP1 alpha antibody 10320-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mechanism of orphan subunit recognition during assembly quality control
Journal: Cell | Authors: Yuichi Yagita | Species: human | Application: WB - EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
Journal: Sci Adv | Authors: Chikako Okubo | Species: human | Application: WB - CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.
Journal: J Cell Biol | Authors: Yu-Cheng Zhang | Species: human | Application: WB - TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis
Journal: Front Cell Dev Biol | Authors: Wen-Shuai Tang | Species: mouse,human | Application: WB - Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid
Journal: J Alzheimers Dis | Authors: Ye Shan | Species: mouse | Application: WB - Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.
Journal: Virology | Authors: Sandhya Gopal | Species: mouse | Application: IP - A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.
Journal: J Agric Food Chem | Authors: Cheng Liu | Species: mouse | Application: WB - CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.
Journal: J Cell Biol | Authors: Yu-Cheng Zhang | Species: human | Application: WB - Crosstalk of RNA methylation writers defines tumor microenvironment and alisertib resistance in breast cancer
Journal: Front Endocrinol (Lausanne) | Authors: Xiaoqiang Zhang | Species: human | Application: WB - TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis
Journal: Front Cell Dev Biol | Authors: Wen-Shuai Tang | Species: mouse,human | Application: WB - METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression
Journal: Cell Signal | Authors: Mengmeng Zhang | Species: human | Application: WB,CoIP - Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid
Journal: J Alzheimers Dis | Authors: Ye Shan | Species: mouse | Application: WB - Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.
Journal: Virology | Authors: Sandhya Gopal | Species: mouse | Application: IP
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