GLI2-Specific Polyclonal antibody 18989-1-AP proteintech
$149.00
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SKU
18989-1-AP
| Catalog Number | 18989-1-AP |
| Synonyms | Gli2, AW546128, Gli 2, GLI family zinc finger 2, GLI Kruppel family member GLI2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, chamaeleo calyptratus |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, HEK-293 cells, human testis tissue |
| Tested Applications — Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, HEK-293 cells, human testis tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chamaeleo calyptratus |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | GLI family zinc finger 2 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 167 kDa， 88 kDa |
| GenBank Accession Number | NM_005270 |
| Gene Symbol | GLI2 |
| Gene ID (NCBI) | 2736 |
| RRID | AB_10596479 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GLI2-Specific antibody 18989-1-AP | Download protocol |
| IHC protocol for GLI2-Specific antibody 18989-1-AP | Download protocol |
| WB protocol for GLI2-Specific antibody 18989-1-AP | Download protocol |
| mouse | WB Acta Pharmacol Sin 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia Authors - Xiao-Wei Li View Article |
| human | WB Front Cell Dev Biol Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway. Authors - Wanshuang Cao View Article |
| Citations | 64 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively. Protocols Product Specific Protocols IF protocol for GLI2-Specific antibody 18989-1-AP Download protocol IHC protocol for GLI2-Specific antibody 18989-1-AP Download protocol WB protocol for GLI2-Specific antibody 18989-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation
Journal: Cell Death Dis | Authors: Pei-Rung Wu | Species: mouse | Application: WB - Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.
Journal: J Cell Biol | Authors: Ivona Mateska | Species: human | Application: WB - Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.
Journal: Elife | Authors: Christina R Adams KD Validated | Species: human | Application: WB,IHC,IF - 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia
Journal: Acta Pharmacol Sin | Authors: Xiao-Wei Li | Species: mouse | Application: WB - HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.
Journal: Cell Mol Life Sci | Authors: Uma Thanigai Arasu | Species: human | Application: WB - Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.
Journal: Front Cell Dev Biol | Authors: Wanshuang Cao | Species: human | Application: WB - MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types.
Journal: Int J Biol Sci | Authors: Xiaolin Wang | Species: mouse | Application: WB,IHC - TGF-β1/SMAD3-mediated Non-canonical Hedgehog Signaling Promotes Pancreatic Stellate Cell Activation and Fibrosis in Chronic Pancreatitis
Journal: Int J Biol Sci | Authors: Linrui Peng | Species: mouse | Application: WB,IP,IHC,IF,CoIP - A Sonic Hedgehog-Gli-Bmi1 signaling pathway plays a critical role in p27 deficiency induced bone anabolism.
Journal: Int J Biol Sci | Authors: Jun Wu | Species: mouse | Application: WB,IHC - Rosmarinic Acid Decreases the Malignancy of Pancreatic Cancer Through Inhibiting Gli1 Signaling.
Journal: Phytomedicine | Authors: Xiang Zhou | Species: human | Application: WB - Targeted inhibition of the HNF1A/SHH axis by triptolide overcomes paclitaxel resistance in non-small cell lung cancer
Journal: Acta Pharmacol Sin | Authors: Ling-Bing Li | Species: human | Application: WB - 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia
Journal: Acta Pharmacol Sin | Authors: Xiao-Wei Li | Species: mouse | Application: WB - P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression
Journal: Leukemia | Authors: Quanfu Li | Species: mouse,human | Application: WB - Curcumin Suppresses Lung Cancer Stem Cells via Inhibiting Wnt/β-catenin and Sonic Hedgehog Pathways.
Journal: Phytother Res | Authors: Jian-Yun Zhu | Species: mouse | Application: WB - Merlin controls limb development and thumb formation by regulating primary cilium-hedgehog signaling
Journal: Cell Rep | Authors: Shali Wu | Species: mouse | Application: WB - Low-dose phthalates promote breast cancer stem cell properties via the oncogene ΔNp63α and the Sonic hedgehog pathway
Journal: Ecotoxicol Environ Saf | Authors: Wan-Shuang Cao | Species: human | Application: WB - HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells.
Journal: Cell Mol Life Sci | Authors: Uma Thanigai Arasu | Species: human | Application: WB - Range of SHH signaling in adrenal gland is limited by membrane contact to cells with primary cilia.
Journal: J Cell Biol | Authors: Ivona Mateska | Species: human | Application: WB - Jujuboside A Regulates Calcium Homeostasis and Structural Plasticity to Alleviate Depression-Like Behavior via Shh Signaling in Immature Neurons
Journal: Drug Des Devel Ther | Authors: Ziyan Zhong | Species: rat | Application: WB,IF - Carnitine Protects against MPP+-Induced Neurotoxicity and Inflammation by Promoting Primary Ciliogenesis in SH-SY5Y Cells
Journal: Cells | Authors: Ji-Eun Bae | Species: human | Application: WB - Cigarette smoke stimulates the stemness of renal cancer stem cells via Sonic Hedgehog pathway.
Journal: Oncogenesis | Authors: Weiwei Qian | Species: human | Application: WB - Curcumin and Methotrexate: A Promising Combination for Osteosarcoma Treatment via Hedgehog Pathway Inhibition
Journal: Int J Mol Sci | Authors: Giulia Giliberti | Species: human | Application: WB - Germacrone promotes diabetic ulcer healing via Hedgehog pathway activation.
Journal: Int Immunopharmacol | Authors: Yun-Xi Cai - Ablated Sonic Hedgehog Signaling in the Dentate Gyrus of the Dorsal and Ventral Hippocampus Impairs Hippocampal-Dependent Memory Tasks and Emotion in a Rat Model of Depression
Journal: Mol Neurobiol | Authors: Yan Luo | Species: rat | Application: WB,IF - Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.
Journal: Front Cell Dev Biol | Authors: Wanshuang Cao | Species: human | Application: WB - Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOG.
Journal: Mol Med Rep | Authors: Huan Zhao | Species: human | Application: WB,IHC - Disrupting mitochondrial β-oxidation by depletion of HADHA impairs primary ciliogenesis.
Journal: Sci Rep | Authors: Joon Bum Kim | Species: human | Application: WB - Identification of diagnostic and therapeutic roles of programmed cell death-related proteins in dilated cardiomyopathy: a multi-omics and experimental validation study.
Journal: Sci Rep | Authors: Jili Fan - Stable overexpression of Nup88 enhances HeLa cell migration through independent regulation of Kif7 and Gli1.
Journal: Oncol Rep | Authors: Masaki Makise - Combined inhibition of sonic Hedgehog signaling and histone deacetylase is an effective treatment for liver cancer.
Journal: Oncol Rep | Authors: Jingmin Li | Species: human | Application: WB - Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.
Journal: Biomed Environ Sci | Authors: Qian Zhou - Inhibition of MicroRNA-195 Alleviates Neuropathic Pain by Targeting Patched1 and Inhibiting SHH Signaling Pathway Activation.
Journal: Neurochem Res | Authors: Xuhui Wang | Species: rat | Application: WB - Identification by RNA-Seq of let-7 clusters as prenatal biomarkers for nonsyndromic cleft lip with palate.
Journal: Ann N Y Acad Sci | Authors: Shanshan Jia | Species: human | Application: WB - Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.
Journal: Acta Biochim Biophys Sin (Shanghai) | Authors: Libin Song | Species: human | Application: WB - MicroRNA-22-3p suppresses hair regrowth in androgenetic alopecia by targeting chloride intracellular channel 4
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Haijing Fu | Application: WB - Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.
Journal: J Cell Physiol | Authors: Caleb S Hayes | Species: mouse | Application: WB - Meningitic Escherichia coli α-hemolysin aggravates blood-brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling.
Journal: Mol Brain | Authors: Jiyang Fu | Species: mouse | Application: WB,IF - Enhanced primary ciliogenesis via mitochondrial oxidative stress activates AKT to prevent neurotoxicity in HSPA9/mortalin-depleted SH-SY5Y cells
Journal: Mol Brain | Authors: Ji-Eun Bae | Species: human | Application: WB - Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.
Journal: Int J Food Sci Nutr | Authors: Miaomiao Ge | Species: human | Application: WB - Astrocyte-Derived TGFβ1 Facilitates Blood-Brain Barrier Function via Non-Canonical Hedgehog Signaling in Brain Microvascular Endothelial Cells.
Journal: Brain Sci | Authors: Jiyang Fu | Species: mouse,human | Application: WB,IF - Decreased Indian hedgehog signaling activates autophagy in endometriosis and adenomyosis.
Journal: Reproduction | Authors: Yingying Zhou | Species: human | Application: WB - CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma.
Journal: J Cancer | Authors: Jiakai Mao | Species: human | Application: WB - Astrocyte-Derived TGFβ1 Facilitates Blood-Brain Barrier Function via Non-Canonical Hedgehog Signaling in Brain Microvascular Endothelial Cells.
Journal: Brain Sci | Authors: Jiyang Fu | Species: mouse,human | Application: WB,IF - Multifaceted approach toward mapping out the anticancer properties of small molecules via in vitro evaluation on melanoma and nonmelanoma skin cancer cells, and in silico target fishing
Journal: Chem Biol Drug Des | Authors: Samuel T Boateng | Species: mouse,human | Application: WB - GLI family zinc finger protein 2 promotes skin fibroblast proliferation and DNA damage repair by targeting the miR-200/ataxia telangiectasia mutated axis in diabetic wound healing
Journal: Kaohsiung J Med Sci | Authors: Zun-Hong Liang | Species: human | Application: WB - Expression patterns of key Sonic Hedgehog signaling pathway components in the developing and adult mouse midbrain and in the MN9D cell line.
Journal: Cell Tissue Res | Authors: Melanie Feuerstein | Species: mouse | Application: WB,IF - SURF4 inhibits autophagy to enhance lung adenocarcinoma progression and stemness through Hedgehog pathway regulation.
Journal: Tissue Cell | Authors: Junzheng Zhou - Disulfiram/copper targets stem cell-like ALDH+ population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.
Journal: J Cell Biochem | Authors: Na Jin | Species: human | Application: WB - Inhibition of Hedgehog Signaling Ameliorates Severity of Chronic Pancreatitis in Experimental Mouse Models
Journal: Am J Physiol Gastrointest Liver Physiol | Authors: Srikanth Iyer | Species: human | Application: IF - Disulfiram/copper targets stem cell-like ALDH+ population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway.
Journal: J Cell Biochem | Authors: Na Jin | Species: human | Application: WB - The anti-tumor activity of tangeretin in esophageal squamous cell carcinoma by inhibiting GLI2-mediated transcription of GPNMB
Journal: PLoS One | Authors: Dong Yang | Species: human | Application: WB - Integrated prognostic assessment of apoptosis and chemotherapy related gene in bladder cancer: a prognostic signature
Journal: Discov Oncol | Authors: Keyuan Zhao | Species: human | Application: WB - Primary Cilia Negatively Regulate Melanogenesis in Melanocytes and Pigmentation in a Human Skin Model.
Journal: PLoS One | Authors: Hyunjung Choi | Species: human | Application: WB - Resveratrol Inhibition of Renal Cancer Stem Cell Characteristics and Modulation of the Sonic Hedgehog Pathway.
Journal: Nutr Cancer | Authors: Hongliang Sun | Species: human | Application: WB - Molecular mechanism of angiogenesis for cerebral infarction rats by acupuncture intervention based on sonic hedgehog signaling pathway
Journal: Physiol Behav | Authors: Huan-Nan Wu | Species: rat | Application: IHC - Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction.
Journal: Biochem Biophys Res Commun | Authors: Pai Pang | Species: mouse | Application: IHC,IF - The GLI2/CDH6 axis enhances migration, invasion and mitochondrial fission of stomach adenocarcinoma cells
Journal: Biochem Biophys Res Commun | Authors: Guang-Yi Liu | Species: human | Application: WB - Curcumin inhibits bladder cancer stem cells by suppressing Sonic Hedgehog pathway.
Journal: Biochem Biophys Res Commun | Authors: Dengdian Wang | Species: human | Application: WB - Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells.
Journal: Oncol Lett | Authors: Enlai Li | Species: human | Application: WB - Benzidine promotes the stemness of bladder cancer stem cells via activation of the Sonic hedgehog pathway.
Journal: Oncol Lett | Authors: Dengdian Wang | Species: human | Application: WB - miR-384-5p targets Gli2 and negatively regulates age-related osteogenic differentiation of rat bone marrow mesenchymal stem cells.
Journal: Stem Cells Dev | Authors: Xiaoming Li | Species: rat | Application: WB - Developmental mechanisms driving complex tooth shape in reptiles.
Journal: Dev Dyn | Authors: Marie Landova Sulcova | Species: Chamaeleo calyptratus | Application: IF - Cigarette smoke supports stemness and epithelial-mesenchymal transition in bladder cancer stem cells through SHH signaling.
Journal: Int J Clin Exp Pathol | Authors: Xianchao Sun | Species: human | Application: WB - Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.
Journal: Elife | Authors: Christina R Adams | Species: human | Application: WB,IHC,IF
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