THRAP3 Polyclonal antibody 19744-1-AP proteintech
$149.00
In stock
SKU
19744-1-AP
| Catalog Number | 19744-1-AP |
| Synonyms | TRAP150, Thyroid hormone receptor-associated protein complex 150 kDa component, Thyroid hormone receptor-associated protein 3, BCLAF2, BCLAF1 and THRAP3 family member 2 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, LNCaP cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human ovary tumor tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | HeLa cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | thyroid hormone receptor associated protein 3 |
| Calculated Molecular Weight | 109 kDa |
| Observed Molecular Weight | 109-130 kDa |
| GenBank Accession Number | NM_005119 |
| Gene Symbol | THRAP3 |
| Gene ID (NCBI) | 9967 |
| RRID | AB_11232035 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2W1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IHC protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| IP protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| WB protocol for THRAP3 antibody 19744-1-AP | Download protocol |
| pig | WB Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. Authors - Xin Zong View Article |
| human | WB,CoIP PLoS Biol CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy. Authors - Xincheng Gao View Article |
| Citations | 6 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
THRAP3, also named as TRAP150, plays a role in transcriptional coactivation. The antibody recognizes the C-term of THRAP3. Protocols Product Specific Protocols IF protocol for THRAP3 antibody 19744-1-AP Download protocol IHC protocol for THRAP3 antibody 19744-1-AP Download protocol IP protocol for THRAP3 antibody 19744-1-AP Download protocol WB protocol for THRAP3 antibody 19744-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.
Journal: Nucleic Acids Res | Authors: Xin Zong | Species: pig | Application: WB - Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.
Journal: J Cell Sci | Authors: Valentina Iadevaia | Application: IF - NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer
Journal: Cancer Res | Authors: Ruihui Xie | Species: human | Application: WB - NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing
Journal: Commun Biol | Authors: Zhongliang Chen | Species: human | Application: WB,IF,CoIP - Deciphering the clinical significance and kinase functions of GSK3α in colon cancer by proteomics and phosphoproteomics
Journal: Mol Cell Proteomics | Authors: Li Gao | Species: human | Application: WB,CoIP - CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy.
Journal: PLoS Biol | Authors: Xincheng Gao | Species: human | Application: WB,CoIP
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