CoraLite®555-conjugated TIA1 Polyclonal antibody CL555-12133 proteintech
$479.00
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SKU
CL555-12133
| Catalog Number | CL555-12133 |
| Synonyms | Nucleolysin TIA 1 isoform p40, p40 TIA 1, RNA binding protein TIA 1, TIA 1, TIA1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Positive IF/ICC detected in | HepG2 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2778 Product name: Recombinant human TIA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC015944 Sequence: MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRKIMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYECRCIGEEKEMWNFGEKYARF Predict reactive species |
| Full Name | TIA1 cytotoxic granule-associated RNA binding protein |
| Calculated Molecular Weight | 214 aa, 24 kDa, 43 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC015944 |
| Gene Symbol | TIA1 |
| Gene ID (NCBI) | 7072 |
| RRID | AB_2923891 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
| Excitation Laser | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31483 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL555 TIA1 antibody CL555-12133 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:300-1:1200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | CoraLite®555 |
TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa. one of these was a missense variant (P362L) in TIA1. Similar to the ALS-related disease proteins TDP-43, hnRNPA1, and FUS, TIA1 is an RNA-binding protein containing a prionlike LCD and assembles into membrane-less organelles, including SGs. Postmortem neuropathology of five TIA1mutations carriers showed a consistent pathological signature with numerous round, hyaline, TAR DNA-binding protein 43 (TDP-43)-positive inclusions.TIA1mutations significantly increased the propensity of TIA1 protein to undergo phase transition. In live cells,TIA1mutations delayed stress granule (SG) disassembly and promoted the accumulation of non-dynamic SGs that harbored TDP-43. Moreover, TDP-43 in SGs became less mobile and insoluble. Protocols Product Specific Protocols IF protocol for CL555 TIA1 antibody CL555-12133 Download protocol Standard Protocols Click here to view our Standard Protocols
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