TK1 Polyclonal antibody 15691-1-AP proteintech
$149.00
In stock
SKU
15691-1-AP
| Catalog Number | 15691-1-AP |
| Synonyms | EC:2.7.1.21, TK 1, TK 2, Tk1a, Tk1b |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, MCF-7 cells, SW 1990 cells |
| Tested Applications — Positive IP detected in | HEK-293 cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, MCF-7 cells, SW 1990 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8249 Product name: Recombinant human TK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC007986 Sequence: MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN Predict reactive species |
| Full Name | thymidine kinase 1, soluble |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC007986 |
| Gene Symbol | TK1 |
| Gene ID (NCBI) | 7083 |
| RRID | AB_2203613 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04183 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TK1 antibody 15691-1-AP | Download protocol |
| IHC protocol for TK1 antibody 15691-1-AP | Download protocol |
| IP protocol for TK1 antibody 15691-1-AP | Download protocol |
| WB protocol for TK1 antibody 15691-1-AP | Download protocol |
| mouse,human | WB,IHC Cell Metab Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners Authors - Qing Li KD Validated View Article |
| rat | IF Theranostics Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist. Authors - Lijian Zhang View Article |
| human | WB Oxid Med Cell Longev lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients. Authors - Guibin Liang View Article |
| Citations | 19 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264). Protocols Product Specific Protocols IF protocol for TK1 antibody 15691-1-AP Download protocol IHC protocol for TK1 antibody 15691-1-AP Download protocol IP protocol for TK1 antibody 15691-1-AP Download protocol WB protocol for TK1 antibody 15691-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners
Journal: Cell Metab | Authors: Qing Li KD Validated | Species: mouse,human | Application: WB,IHC - Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist.
Journal: Theranostics | Authors: Lijian Zhang | Species: rat | Application: IF - ERK-USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth
Journal: Nat Struct Mol Biol | Authors: Jingjing Tao | Species: human | Application: IHC - Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer
Journal: Clin Transl Med | Authors: Xin Sun | Species: human | Application: WB - Proteomics analysis and immune profiling reveal regulators of PD-L1 in oesophageal squamous cell carcinoma
Journal: Br J Cancer | Authors: Yuying Li | Species: human | Application: WB - lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.
Journal: Oxid Med Cell Longev | Authors: Guibin Liang | Species: human | Application: WB - Discovery of Novel Oxazolo[4,3-f]purine Derivatives as Antitumor Agents through PPIA Interaction
Journal: J Med Chem | Authors: Xian-Jia Li | Species: human | Application: WB - Thymidine kinase 1 related to Prolif-like T cells promoted GBM through regulation of cell cycle and EMT signals: a comprehensive research based on multi-omics analysis and experimental validation
Journal: Front Immunol | Authors: Wentao Deng - A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth
Journal: PLoS Biol | Authors: Iratxe Zuazo-Gaztelu | Species: human | Application: WB - rAAV9-UPII-TK-EGFP can precisely transduce a suicide gene and inhibit the growth of bladder tumors.
Journal: Cancer Biol Ther | Authors: Foyan Lian | Species: mouse,human | Application: WB - Neutrophil extracellular traps accelerate vascular smooth muscle cell proliferation via Akt/CDKN1b/TK1 accompanying with the occurrence of hypertension
Journal: J Hypertens | Authors: Xinhui Fang | Species: rat | Application: WB - Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.
Journal: J Cell Mol Med | Authors: Xu Jiang | Species: human | Application: WB - Thymidine Kinase 1 Mediates the Synergistic Antitumor Activity of Ubenimex and Celecoxib via Regulation of Cell Cycle in Colorectal Cancer
Journal: J Pharmacol Exp Ther | Authors: Aimin Wang | Species: mouse,human | Application: WB - KAT7-acetylated YBX1 promotes hepatocellular carcinoma proliferation by reprogramming nucleotide metabolism
Journal: BMC Cancer | Authors: He Huang | Species: human | Application: WB,IHC - All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain
Journal: ACS Pharmacol Transl Sci | Authors: Hannah N Wilkins | Species: human | Application: WB - Elimination of radiation-induced senescent cancer cells and stromal cells in vitro by near-infrared photoimmunotherapy
Journal: Cancer Med | Authors: Motofumi Suzuki | Species: human | Application: WB - Potential tumor-specific antigens and immune landscapes identification for mRNA vaccine in thyroid cancer
Journal: Front Oncol | Authors: Xiaoning Wang | Species: human | Application: IHC - Fhit and Wwox loss-associated genome instability: A genome caretaker one-two punch.
Journal: Adv Biol Regul | Authors: Morgan S Schrock | Species: mouse | Application: WB - A human stem cell model of neuroblastoma identifies four chromosome 17q genes that drive tumor initiation and progression.
Journal: Cancer Lett | Authors: Wanqi Fang
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