TLE3 Polyclonal antibody 22094-1-AP proteintech
$149.00
In stock
SKU
22094-1-AP
| Catalog Number | 22094-1-AP |
| Synonyms | HsT18976, GRG3, GRG 3, ESG3, ESG |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, A431 cells, HepG2 cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Positive WB detected in | HEK-293 cells, A431 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17300 Product name: Recombinant human TLE3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 158-240 aa of BC015729 Sequence: PVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDS Predict reactive species |
| Full Name | transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) |
| Calculated Molecular Weight | 772 aa, 83 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC015729 |
| Gene Symbol | TLE3 |
| Gene ID (NCBI) | 7090 |
| RRID | AB_2878993 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04726 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TLE3 antibody 22094-1-AP | Download protocol |
| IHC protocol for TLE3 antibody 22094-1-AP | Download protocol |
| IP protocol for TLE3 antibody 22094-1-AP | Download protocol |
| WB protocol for TLE3 antibody 22094-1-AP | Download protocol |
| mouse | WB,IF Cells Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development. Authors - Sangho Lee KD Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:300-1:1200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES. Protocols Product Specific Protocols IF protocol for TLE3 antibody 22094-1-AP Download protocol IHC protocol for TLE3 antibody 22094-1-AP Download protocol IP protocol for TLE3 antibody 22094-1-AP Download protocol WB protocol for TLE3 antibody 22094-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Differential Regulation of TLE3 in Sertoli Cells of the Testes during Postnatal Development.
Journal: Cells | Authors: Sangho Lee KD Validated | Species: mouse | Application: WB,IF - TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry
Journal: J Cell Sci | Authors: Jonathan Kelebeev
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