CoraLite® Plus 750-conjugated TNFR2/CD120b Recombinant monoclonal antibody CL750-83101 proteintech
$479.00
In stock
SKU
CL750-83101
| Catalog Number | CL750-83101 |
| Synonyms | TNFRSF1B, 230328B6, CD120b, Etanercept, p75 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | WB |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | THP-1 cells, K-562 cells, U-937 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Positive WB detected in | THP-1 cells, K-562 cells, U-937 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg0086 Product name: Recombinant Human TNFR2/CD120b protein (Myc Tag, His Tag) Source: mammalian cells -derived, pHZ-KIsec Tag: Myc & 6*His Domain: 23-257 aa of BC052977 Sequence: LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGD Predict reactive species |
| Full Name | tumor necrosis factor receptor superfamily, member 1B |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 75 kDa, 65 kDa |
| GenBank Accession Number | BC052977 |
| Gene Symbol | TNFR2 |
| Gene ID (NCBI) | 7133 |
| RRID | AB_3673766 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Excitation Laser | Red Laser (633 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P20333 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| WB protocol for CL Plus 750 TNFR2/CD120b antibody CL750-83101 | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230328B6 |
| Conjugation | CoraLite® Plus 750 |
Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699). Protocols Product Specific Protocols WB protocol for CL Plus 750 TNFR2/CD120b antibody CL750-83101 Download protocol Standard Protocols Click here to view our Standard Protocols
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