NONO Polyclonal antibody 11058-1-AP proteintech
$149.00
In stock
SKU
11058-1-AP
| Catalog Number | 11058-1-AP |
| Synonyms | 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, NonO protein, Non-POU domain-containing octamer-binding protein, NRB54 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, zebrafish, bovine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | PC-3 cells, human pancreas tissue, human skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, HEK-293 cells, PC-12 cells, NIH/3T3 cells |
| Tested Applications — Positive IP detected in | HEK-293T cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HEK-293 cells, HCT 116 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | PC-3 cells, human pancreas tissue, human skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, HEK-293 cells, PC-12 cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, HCT 116 cells |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, bovine |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1512 Product name: Recombinant human NONO protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 124-471 aa of BC010049 Sequence: IAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY Predict reactive species |
| Full Name | non-POU domain containing, octamer-binding |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC010049 |
| Gene Symbol | NONO |
| Gene ID (NCBI) | 4841 |
| RRID | AB_2152167 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for NONO antibody 11058-1-AP | Download protocol |
| IHC protocol for NONO antibody 11058-1-AP | Download protocol |
| IP protocol for NONO antibody 11058-1-AP | Download protocol |
| WB protocol for NONO antibody 11058-1-AP | Download protocol |
| human | WB Nat Aging Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling Authors - Donghong Cai View Article |
| zebrafish | WB Nat Genet Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human Authors - Wenze Huang View Article |
| Roy (Verified Customer) (06-28-2024) | Perfect antibody to detect NONO by WB (Overnight incubation at 4°C, 1/1000 dilution) Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: HeLa and Jurkat cells |
| Xingyuan (Verified Customer) (07-17-2023) | The band quality was decent. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: 293T NONO Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11058-1-AP) |
| Tatyana (Verified Customer) (05-14-2023) | ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: HeLa NONO Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11058-1-AP) |
| Citations | 66 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation. Protocols Product Specific Protocols IF protocol for NONO antibody 11058-1-AP Download protocol IHC protocol for NONO antibody 11058-1-AP Download protocol IP protocol for NONO antibody 11058-1-AP Download protocol WB protocol for NONO antibody 11058-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
Journal: Cell | Authors: Lei Sun - Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.
Journal: Cell Res | Authors: Shaojun Zhang | Species: human | Application: WB - Combating Atherosclerosis with Chirality/Phase Dual-Engineered Nanozyme Featuring Microenvironment-Programmed Senolytic and Senomorphic Actions
Journal: Adv Mater | Authors: Chengjin Ding | Species: human | Application: IF - Proline exacerbates hepatic gluconeogenesis via paraspeckle-dependent mRNA retention
Journal: Nat Metab | Authors: Yurong Zhao | Species: human | Application: WB,RIP - Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human
Journal: Nat Genet | Authors: Wenze Huang | Species: zebrafish | Application: WB - Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling
Journal: Nat Aging | Authors: Donghong Cai | Species: human | Application: WB
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