TOX2 Polyclonal antibody 21162-1-AP proteintech
$149.00
In stock
SKU
21162-1-AP
| Catalog Number | 21162-1-AP |
| Synonyms | GCX-1, GCX1, C20orf100 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ChIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, rat liver tissue, MCF-7 cells, mouse liver tissue |
| Tested Applications — Positive IP detected in | mouse liver tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | A549 cells, rat liver tissue, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag14772 Product name: Recombinant human TOX2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 303-488 aa of BC007636 Sequence: SSPDQGETKSTQANPPAKMLPPKQPMYAMPGLASFLTPSDLQAFRSGASPASLARTLGSKSLLPGLSASPPPPPSFPLSPTLHQQLSLPPHAQGALLSPPVSMSPAPQPPVLPTPMALQVQLAMSPSPPGPQDFPHISEFPSSSGSCSPGPSNPTSSGDWDSSYPSGECGISTCSLLPRDKSLYLT Predict reactive species |
| Full Name | TOX high mobility group box family member 2 |
| Calculated Molecular Weight | 488 aa, 52 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007636 |
| Gene Symbol | TOX2 |
| Gene ID (NCBI) | 84969 |
| RRID | AB_2878821 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NM4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IP protocol for TOX2 antibody 21162-1-AP | Download protocol |
| WB protocol for TOX2 antibody 21162-1-AP | Download protocol |
| mouse | IHC Cell Commun Signal Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulation. Authors - Laura D. McGee View Article |
| human | WB,IF,ChIP Mol Cancer Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma Authors - Jianbiao Zhou KD Validated View Article |
| Edma (Verified Customer) (03-03-2025) | The antibody works very well Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1:1000 or 1:200 Cell Tissue Type: mouse lung tissue TOX2 Antibody Western Blot,Immunofluorescence validation (1:1000 or 1:200 dilution) in mouse lung tissue (Cat no:21162-1-AP) |
| Citations | 4 |
| Dilutions | WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127). Protocols Product Specific Protocols IHC protocol for TOX2 antibody 21162-1-AP Download protocol IP protocol for TOX2 antibody 21162-1-AP Download protocol WB protocol for TOX2 antibody 21162-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells.
Journal: Sci Adv | Authors: Shu Horiuchi KO Validated | Species: mouse | Application: WB - Multimodal features and prognostic risk assessment in locally advanced gastric cancer patients following neoadjuvant therapy based on machine learning algorithms: a multicenter study.
Journal: Gastric Cancer | Authors: Li Liu - Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma
Journal: Mol Cancer | Authors: Jianbiao Zhou KD Validated | Species: human | Application: WB,IF,ChIP - Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulation.
Journal: Cell Commun Signal | Authors: Laura D. McGee | Species: mouse | Application: IHC
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