TRIM17 Polyclonal antibody 13663-1-AP proteintech
$149.00
In stock
SKU
13663-1-AP
| Catalog Number | 13663-1-AP |
| Synonyms | E3 ubiquitin-protein ligase TRIM17, EC:2.3.2.27, RBCC, RING-type E3 ubiquitin transferase TRIM17, RNF16 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, COLO 320 cells, mouse spleen tissue, mouse thymus tissue |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, COLO 320 cells, mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag4572 Product name: Recombinant human TRIM17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 177-477 aa of BC033788 Sequence: KVKERRERIVLEFEKMNLYLVEEEQRLLQALETEEEETASRLRESVACLDRQGHSLELLLLQLEERSTQGPLQMLQDMKEPLSRKNNVSVQCPEVAPPTRPRTVCRVPGQIEVLRGFLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPFFCLGAPKSGQMVISTVTMWVKG Predict reactive species |
| Full Name | tripartite motif-containing 17 |
| Calculated Molecular Weight | 477 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC033788 |
| Gene Symbol | TRIM17 |
| Gene ID (NCBI) | 51127 |
| RRID | AB_10644443 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y577 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TRIM17 antibody 13663-1-AP | Download protocol |
| IHC protocol for TRIM17 antibody 13663-1-AP | Download protocol |
| WB protocol for TRIM17 antibody 13663-1-AP | Download protocol |
| human | WB,IHC Cell Death Dis ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA. Authors - Shuhao Wang View Article |
| human,mouse | WB J Cell Mol Med Jianpi Jiedu Xiaozheng Fang Regulates Hepatocellular Carcinoma Proliferation and Metastasis Based on Network Pharmacology. Authors - Bin Li View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TRIM17 (Tripartite motif-containing 17) also known as RNF16 (Ring Finger protein 16) or terf (testis RING finger protein), is a member of the TRIM family, a large class of RING-containing E3 ubiquitin-ligases. It is expressed at low levels in adult tissues, except in testis and in some brain regions (PMID: 34069831). TRIM17 contributes to gastric cancer survival through regulating BAX protein stability and antagonizing apoptosis, which provides a promising therapeutic target for GC treatment and a biomarker for prognosis (PMID: 27562068, 37697039). Protocols Product Specific Protocols IF protocol for TRIM17 antibody 13663-1-AP Download protocol IHC protocol for TRIM17 antibody 13663-1-AP Download protocol WB protocol for TRIM17 antibody 13663-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- TRIM17 downregulation modulates CRPC progression and enzalutamide resistance by derepressing BCL2 expression via the p53-dependent and p53-independent pathways.
Journal: Cell Death Differ | Authors: Zhi Shang - The E3 ubiquitin ligase TRIM17 promotes gastric cancer survival and progression via controlling BAX stability and antagonizing apoptosis
Journal: Cell Death Differ | Authors: Jiajia Shen KD Validated | Species: human | Application: WB,IHC - Jianpi Jiedu Xiaozheng Fang Regulates Hepatocellular Carcinoma Proliferation and Metastasis Based on Network Pharmacology.
Journal: J Cell Mol Med | Authors: Bin Li | Species: human,mouse | Application: WB - ILF3 promotes colorectal cancer cell resistance to ferroptosis by enhancing cysteine uptake and GSH synthesis via stabilizing SLC3A2 mRNA.
Journal: Cell Death Dis | Authors: Shuhao Wang | Species: human | Application: WB,IHC - Fumarate hydratase suppresses glioblastoma progression by restricting fumarate-mediated succination of TRIM17.
Journal: J Biol Chem | Authors: Qianqian Ju
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