TRIM46 Polyclonal antibody 21026-1-AP proteintech
$149.00
In stock
SKU
21026-1-AP
| Catalog Number | 21026-1-AP |
| Synonyms | GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46, Tripartite, fibronectin type-III and C-terminal SPRY motif protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA |
| Applications | WB, IF/ICC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, mouse brain tissue, Jurkat cells, rat brain tissue |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, mouse brain tissue, Jurkat cells, rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | A549 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag15290 Product name: Recombinant human TRIM46 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC069416 Sequence: MAEGEDMQTFTSIMDALVRISTSMKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSRRTLPKPDRLDRLLKSGFGTYPGRKRGALHPQVIMFPCPACQGDVELGERGLAGLFRNLTLERVVERYRQSVSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEEVTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLTYILGNQDTVQTQICELEEAVRHTEVSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLARLSAQI Predict reactive species |
| Full Name | tripartite motif-containing 46 |
| Calculated Molecular Weight | 759 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC069416 |
| Gene Symbol | TRIM46 |
| Gene ID (NCBI) | 80128 |
| RRID | AB_10732843 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z4K8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TRIM46 antibody 21026-1-AP | Download protocol |
| WB protocol for TRIM46 antibody 21026-1-AP | Download protocol |
| mouse | WB,IF Cell Rep The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling. Authors - Sotaro Ichinose View Article |
| human | WB,IF Int J Mol Sci Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46. Authors - Li Zhang View Article |
| Citations | 13 |
| Dilutions | WB : 1:1000-1:4000 IF-P : 1:200-1:800 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa). Protocols Product Specific Protocols IF protocol for TRIM46 antibody 21026-1-AP Download protocol WB protocol for TRIM46 antibody 21026-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.
Journal: Nat Commun | Authors: John K Vuong | Species: mouse | Application: WB,IP - The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.
Journal: Cell Rep | Authors: Sotaro Ichinose | Species: mouse | Application: WB,IF - Comprehensive analysis of ferroptosis-related genes indicates that TRIM46 is a novel biomarker and promotes the progression of ovarian cancer via modulating ferroptosis and Wnt signaling pathway
Journal: Am J Cancer Res | Authors: Shuang Liu KD Validated | Species: human | Application: WB - Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR.
Journal: Oncol Res | Authors: Wenwei Jiang | Species: human | Application: IHC - TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination.
Journal: Exp Cell Res | Authors: Jingfa Zhang KD Validated | Species: human | Application: WB - Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46.
Journal: Int J Mol Sci | Authors: Li Zhang | Species: human | Application: WB,IF - Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR.
Journal: Oncol Res | Authors: Wenwei Jiang | Species: human | Application: IHC - Regulation of Axonal Microtubule Polarity Orientation in Different Kinds of Neurons
Journal: FASEB J | Authors: Ankita Patil | Species: rat | Application: WB - TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination.
Journal: Exp Cell Res | Authors: Jingfa Zhang | Species: human | Application: WB - Comprehensive analysis of ferroptosis-related genes indicates that TRIM46 is a novel biomarker and promotes the progression of ovarian cancer via modulating ferroptosis and Wnt signaling pathway
Journal: Am J Cancer Res | Authors: Shuang Liu | Species: human | Application: WB - Ginsenoside RD2 inhibits gastric cancer progression by suppressing TRIM46-mediated DUSP1 ubiquitination and modulating the ERK signaling pathway.
Journal: J Pharmacol Sci | Authors: Jun Jiang - Trim46 knockout impaired neuronal architecture and caused hypoactive behavior in rats
Journal: Dev Dyn | Authors: Feifei Guan | Species: rat | Application: WB,IF,CoIP - A role for CASM in the repair of damaged Golgi architecture
Journal: bioRxiv | Authors: Seeun Oh
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