TRIP4 Polyclonal antibody 12324-1-AP proteintech
$149.00
In stock
SKU
12324-1-AP
| Catalog Number | 12324-1-AP |
| Synonyms | ASC 1, ASC-1, HsT17391, RQT4, Thyroid receptor-interacting protein 4 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HL-60 cells, COLO 320 cells, HeLa cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HL-60 cells, COLO 320 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2987 Product name: Recombinant human TRIP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 281-581 aa of BC012448 Sequence: QVIDDESDYFASDSNQWLSKLERETLQKREEELRELRHASRLSKKVTIDFAGRKILEEENSLAEYHSRLDETIQAIANGTLNQPLTKLDRSSEEPLGVLVNPNMYQSPPQWVDHTGAASQKKAFRSSGFGLEFNSFQHQLRIQDQEFQEGFDGGWCLSVHQPWASLLVRGIKRVEGRSWYTPHRGRLWIAATAKKPSPQEVSELQATYRLLRGKDVEFPNDYPSGCLLGCVDLIDCLSQKQFKEQFPDISQESDSPFVFICKNPQEMVVKFPIKGNPKIWKLDSKIHQGAKKGLMKQNKAV Predict reactive species |
| Full Name | thyroid hormone receptor interactor 4 |
| Calculated Molecular Weight | 581 aa, 66 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC012448 |
| Gene Symbol | TRIP4 |
| Gene ID (NCBI) | 9325 |
| RRID | AB_10646482 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15650 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TRIP4 antibody 12324-1-AP | Download protocol |
| WB protocol for TRIP4 antibody 12324-1-AP | Download protocol |
| mouse,human | WB Genet Med A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy. Authors - Min Ni View Article |
| human | WB bioRxiv The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses. Authors - Shixin Cui KO Validated View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891) Protocols Product Specific Protocols IHC protocol for TRIP4 antibody 12324-1-AP Download protocol WB protocol for TRIP4 antibody 12324-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.
Journal: Genet Med | Authors: Min Ni | Species: mouse,human | Application: WB - The m6A demethylase FTO suppresses perivascular adipose tissue browning and exacerbates vascular injury via m6A-mediated destabilization of Irx3 mRNA and the IRX3/UCP1 axis.
Journal: Atherosclerosis | Authors: Xiaoyong Hu - Molecular mechanism of MAFB transcriptional activation of PPARD in regulating adipose browning and protecting against vascular endothelial cell injury.
Journal: Exp Cell Res | Authors: Xiaoyong Hu - Ski2-like helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.
Journal: Cell Rep | Authors: Shixin Cui | Species: human | Application: WB,IP,IF - The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.
Journal: bioRxiv | Authors: Shixin Cui KO Validated | Species: human | Application: WB
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