TRMT6 Polyclonal antibody 16727-1-AP proteintech
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SKU
16727-1-AP
| Catalog Number | 16727-1-AP |
| Synonyms | tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, RIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, HuH-7 cells, SMMC-7721 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | HepG2 cells, HuH-7 cells, SMMC-7721 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10176 Product name: Recombinant human TRMT6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-497 aa of BC001262 Sequence: MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATACFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQENEDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAALLSERNADGLIVASRFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINLRLSETWLRNYQVLPDRSHPKLLMSGGGGYLLSGFTVAMDNLKADTSLKSNASTLESHETEEPAAKKRKCPESDS Predict reactive species |
| Full Name | tRNA methyltransferase 6 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC001262 |
| Gene Symbol | TRMT6 |
| Gene ID (NCBI) | 51605 |
| RRID | AB_2878306 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJA5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TRMT6 antibody 16727-1-AP | Download protocol |
| IP protocol for TRMT6 antibody 16727-1-AP | Download protocol |
| WB protocol for TRMT6 antibody 16727-1-AP | Download protocol |
| mouse | WB Small Unveiling Novel Biomarkers: Ferroptosis and m1A in the Progression of Nanographene-Induced Lung Fibrosis. Authors - Jiarui Xia View Article |
| human | WB Cancer Lett YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer Authors - Hongyu Chen View Article |
| Estefania (Verified Customer) (05-16-2022) | Very good signal. Tested using simple western (ProteinSimple) Applications: Western Blot Primary Antibody Dilution: 1:20 Cell Tissue Type: HepG2 cell lysate TRMT6 Antibody Western Blot validation (1:20 dilution) in HepG2 cell lysate (Cat no:16727-1-AP) |
| Citations | 15 |
| Dilutions | WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein. Protocols Product Specific Protocols IHC protocol for TRMT6 antibody 16727-1-AP Download protocol IP protocol for TRMT6 antibody 16727-1-AP Download protocol WB protocol for TRMT6 antibody 16727-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis
Journal: Nat Immunol | Authors: Yongbo Liu KO Validated | Species: mouse | Application: WB - N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.
Journal: Nat Commun | Authors: Yanying Wang | Species: mouse | Application: WB, IHC - ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.
Journal: Cell Discov | Authors: Wenjun Kuang | Species: human | Application: WB - ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17
Journal: Acta Pharm Sin B | Authors: Junting Zhang | Species: mouse | Application: WB - YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer
Journal: Cancer Lett | Authors: Hongyu Chen | Species: human | Application: WB - Unveiling Novel Biomarkers: Ferroptosis and m1A in the Progression of Nanographene-Induced Lung Fibrosis.
Journal: Small | Authors: Jiarui Xia | Species: mouse | Application: WB - tRNA m1A modification regulates cholesterol biosynthesis to promote antitumor immunity of CD8+ T cells.
Journal: J Exp Med | Authors: Shan Miao | Species: mouse | Application: WB - N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction
Journal: Cell Death Discov | Authors: Zhangyang Qi | Species: human | Application: WB - TRMT6-directed m(1)A modification initiates lung squamous cell carcinoma via YTHDF3-stabilized cell cycle genes.
Journal: NPJ Precis Oncol | Authors: Weihao Xue | Species: human | Application: WB,IP,IHC - m1A methylase TRMT6 promotes neuroblastoma development by demethylating SST mRNA in an m1A/YTHDF2-dependent manner.
Journal: Br J Cancer | Authors: Xinxin Zhang | Species: human | Application: WB - Quantifying the mRNA epitranscriptome reveals epitranscriptome signatures and roles in cancer
Journal: Cell Mol Life Sci | Authors: Ying Feng | Species: human | Application: WB - TRMT6 mitigates susceptibility and progression of DSS-induced colitis multifacetedly via translational regulation.
Journal: Sci Rep | Authors: Xianli Zhang | Species: mouse | Application: IHC - From palmitoylation to cholesterol metabolism: The hidden mechanism of lung tumorigenesis.
Journal: Cell Signal | Authors: Li Shen | Species: human | Application: WB,RIP,IP,IF - Hypoxia-induced exosomal circNRIP1 activates cancer-associated fibroblasts to promote esophageal squamous cell carcinoma migration and invasion
Journal: BMC Gastroenterol | Authors: Guan'en Qiao | Application: WB - Cotinine Promotes Cancer Cell Proliferation and m(1)A RNA Methylation in Smoking-Associated Lung Cancer by Targeting PLK1/β-Catenin/TRMT6 Axis.
Journal: Environ Health (Wash) | Authors: Panlong Li
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