TSHR Polyclonal antibody 14450-1-AP proteintech
$149.00
In stock
SKU
14450-1-AP
| Catalog Number | 14450-1-AP |
| Synonyms | CHNG1, hTSHR I, LGR3, Thyroid-stimulating hormone receptor, Thyrotropin receptor |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (2) |
| Reactivity | human, mouse, rat, zebrafish, sheep |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | TT cells, HeLa cells, mouse ovary tissue, mouse thymus tissue |
| Tested Applications — Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | TT cells, HeLa cells, mouse ovary tissue, mouse thymus tissue |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish, sheep |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5957 Product name: Recombinant human TSHR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC063613 Sequence: MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLLPLGRKSLSFETQKAPRSSMPS Predict reactive species |
| Full Name | thyroid stimulating hormone receptor |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 87 kDa, 62-70 kDa, 28-30 kDa |
| GenBank Accession Number | BC063613 |
| Gene Symbol | TSHR |
| Gene ID (NCBI) | 7253 |
| RRID | AB_2208263 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16473 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TSHR antibody 14450-1-AP | Download protocol |
| WB protocol for TSHR antibody 14450-1-AP | Download protocol |
| human | WB J Transl Med Serum and thyroid tissue level of let-7b and their correlation with TRAb in Graves' disease. Authors - Xinxin Chen View Article |
| mouse | IF J Hazard Mater Thyroid cancer risk associated with perfluoroalkyl carboxylate exposure: Assessment using a human dermal fibroblast-derived extracellular matrix-based thyroid cancer organoid Authors - Min Heui Yoo View Article |
| Citations | 28 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein hormone produced by thyrotrophs in the anterior pituitary gland. The TSH receptor (TSHR), mainly expressed on the thyroid epithelial cells, is a G protein-coupled receptor (GPCR) of the seven-transmembrane domain family and plays a central role in controlling thyroid cell metabolism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody raised against N-terminal region (1-253 aa) of human TSHR can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718) Protocols Product Specific Protocols IHC protocol for TSHR antibody 14450-1-AP Download protocol WB protocol for TSHR antibody 14450-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Obatoclax and LY3009120 Efficiently Overcome Vemurafenib Resistance in Differentiated Thyroid Cancer.
Journal: Theranostics | Authors: Wei-Jun Wei | Species: human | Application: WB - PCSK9 promotes progression of anaplastic thyroid cancer through E-cadherin endocytosis
Journal: Cell Death Dis | Authors: Yu Zhang | Species: human | Application: WB - Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer
Journal: Cancer Lett | Authors: Zongfu Pan | Species: human | Application: WB - Thyroid cancer risk associated with perfluoroalkyl carboxylate exposure: Assessment using a human dermal fibroblast-derived extracellular matrix-based thyroid cancer organoid
Journal: J Hazard Mater | Authors: Min Heui Yoo | Species: mouse | Application: IF - Serum and thyroid tissue level of let-7b and their correlation with TRAb in Graves' disease.
Journal: J Transl Med | Authors: Xinxin Chen | Species: human | Application: WB - Thyroid-stimulating hormone receptor mediates peripheral-central neuroimmune crosstalk in autoimmune thyroid diseases.
Journal: BMC Med | Authors: Haiyang Zhang - Thyroid-stimulating hormone receptor mediates peripheral-central neuroimmune crosstalk in autoimmune thyroid diseases.
Journal: BMC Med | Authors: Haiyang Zhang - A TSHR-Targeting Aptamer in Monocytes Correlating with Clinical Activity in TAO
Journal: Int J Nanomedicine | Authors: Jiamin Cao | Species: human | Application: IF - TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells.
Journal: Oxid Med Cell Longev | Authors: Jingwen Fan | Species: mouse | Application: IHC - Acute exposure of zebrafish embryo (Danio rerio) to flutolanil reveals its developmental mechanism of toxicity via disrupting the thyroid system and metabolism.
Journal: Environ Pollut | Authors: Miaomiao Teng | Species: zebrafish | Application: WB - Thyroidal expression of ER molecular chaperone GRP170 is required for efficient TSH-mediated thyroid hormone synthesis
Journal: JCI Insight | Authors: Xiaohan Zhang - The impact and potential mechanisms of long noncoding RNA ENST00000521141.1 on human white adipocyte differentiation.
Journal: J Endocrinol | Authors: Qiong Wu | Species: human | Application: WB - The impact and potential mechanisms of long noncoding RNA ENST00000521141.1 on human white adipocyte differentiation.
Journal: J Endocrinol | Authors: Qiong Wu | Species: human | Application: WB - TRx0237 induces apoptosis and enhances anti-PD-1 immunotherapeutic efficacy in anaplastic thyroid Cancer
Journal: Int Immunopharmacol | Authors: Qingyang Ning | Species: human | Application: IF - Sinomenine Hydrochloride Promotes TSHR-Dependent Redifferentiation in Papillary Thyroid Cancer
Journal: Int J Mol Sci | Authors: Jing Zhang | Species: human | Application: WB - Epigenetic Modulation of Thyroid-Stimulating Hormone Receptor Expression in Orbital Fibroblasts From Graves' Ophthalmopathy: Target for Treatment.
Journal: Invest Ophthalmol Vis Sci | Authors: Rajit Chompoowong - HER inhibitor promotes BRAF/MEK inhibitor-induced redifferentiation in papillary thyroid cancer harboring BRAFV600E.
Journal: Oncotarget | Authors: Lingxiao Cheng | Species: human | Application: WB - Patient-Derived Papillary Thyroid Cancer Organoids for Radioactive Iodine Refractory Screening.
Journal: Cancers (Basel) | Authors: Luc H J Sondorp | Species: human | Application: IF - Combined tazemetostat and MAPKi enhances differentiation of papillary thyroid cancer cells harbouring BRAFV600E by synergistically decreasing global trimethylation of H3K27.
Journal: J Cell Mol Med | Authors: Hao Fu | Species: human | Application: WB - Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation
Journal: Environ Toxicol Pharmacol | Authors: Qi Xu | Species: human | Application: WB - Identification and functionalization of thyrotropin receptor antibodies (TRAb) with different antigenic epitopes
Journal: Am J Physiol Endocrinol Metab | Authors: Jingyi Zheng | Species: human | Application: IF - Identification of TSHB gene expression profile in Duolang sheep and its functional role in granulosa cells.
Journal: BMC Genomics | Authors: HuiPing Sun | Species: sheep | Application: WB - Targeting syndecan-2 inhibits papillary thyroid cancer invasiveness and de-differentiation.
Journal: BMC Endocr Disord | Authors: Rui Liu | Species: human | Application: WB - A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway.
Journal: Am J Cancer Res | Authors: Jianlu Song | Species: human | Application: WB - Dominant negative effect of mutated thyroid stimulating hormone receptor (P556L) causes hypothyroidism in C.RF-Tshr(hyt/wild) mice.
Journal: PLoS One | Authors: Endo Toyoshi T | Species: human | Application: WB - Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embryonic stem cells from refractory mouse strains.
Journal: PLoS One | Authors: Ye Shoudong S | Application: IF - Effect of TSH on aromatase expression of ovarian granulosa cells in obese mice
Journal: Hormones (Athens) | Authors: Liping Zhu | Species: mouse | Application: IHC - ITM2A promotes thyroid cancer differentiation through metabolic reprogramming and enhances PD-L1-dependent T-cell responses.
Journal: Clin Exp Med | Authors: Wuhui Sun
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